Design-based stereological methods for estimating numbers of glomerular podocytes

Puelles, Victor G., Douglas-Denton, Rebecca N., Cullen-McEwen, Luise, McNamara, Bridgette J., Salih, Firuz, Li, Jinhua, Hughson, Michael D., Hoy, Wendy E., Nyengaard, Jens R. and Bertram, John F. (2014) Design-based stereological methods for estimating numbers of glomerular podocytes. Annals of Anatomy, 196 1: 48-56. doi:10.1016/j.aanat.2013.04.007

Author Puelles, Victor G.
Douglas-Denton, Rebecca N.
Cullen-McEwen, Luise
McNamara, Bridgette J.
Salih, Firuz
Li, Jinhua
Hughson, Michael D.
Hoy, Wendy E.
Nyengaard, Jens R.
Bertram, John F.
Title Design-based stereological methods for estimating numbers of glomerular podocytes
Journal name Annals of Anatomy   Check publisher's open access policy
ISSN 0940-9602
Publication date 2014
Year available 2013
Sub-type Article (original research)
DOI 10.1016/j.aanat.2013.04.007
Open Access Status
Volume 196
Issue 1
Start page 48
End page 56
Total pages 9
Place of publication Jena, Germany
Publisher Urban und Fischer Verlag
Collection year 2014
Language eng
Abstract The podocyte depletion hypothesis has emerged as a unifying concept in glomerular pathology. According to this hypothesis podocyte depletion may be absolute (decrease in number of healthy mature podocytes), relative (fewer podocytes per unit of glomerular volume) or involve alterations to the specialized podocyte architecture (such as foot process effacement). To study and understand podocyte depletion it is important to be able to accurately and precisely count these cells. Here we present new design-based stereological methods for estimating podocyte number in individual glomeruli of known volume, and in average glomeruli. Both methods involve serial histological sectioning, triple label immunohistochemistry, laser confocal microscopy and cell counting with the optical disector/fractionator.
Keyword Glomerular volume
Immunohistochemistry and confocal microscopy
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Medicine Publications
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