Assessment of freestanding membranes prepared from Antheraea pernyi silk fibroin as a potential vehicle for corneal epithelial cell transplantation

Hogerheyde, Thomas A., Suzuki, Shuko, Stephenson, Sally A., Richardson, Neil A., Chirila, Traian V., Harkin, Damien G. and Bray, Laura J. (2014) Assessment of freestanding membranes prepared from Antheraea pernyi silk fibroin as a potential vehicle for corneal epithelial cell transplantation. Biomedical Materials (Bristol), 9 2: . doi:10.1088/1748-6041/9/2/025016


Author Hogerheyde, Thomas A.
Suzuki, Shuko
Stephenson, Sally A.
Richardson, Neil A.
Chirila, Traian V.
Harkin, Damien G.
Bray, Laura J.
Title Assessment of freestanding membranes prepared from Antheraea pernyi silk fibroin as a potential vehicle for corneal epithelial cell transplantation
Formatted title
 Assessment of freestanding membranes prepared from Antheraea pernyi silk fibroin as a potential vehicle for corneal epithelial cell transplantation
Journal name Biomedical Materials (Bristol)   Check publisher's open access policy
ISSN 1748-6041
1748-605X
Publication date 2014-04-01
Year available 2014
Sub-type Article (original research)
DOI 10.1088/1748-6041/9/2/025016
Volume 9
Issue 2
Total pages 9
Place of publication Bristol, United Kingdom
Publisher Institute of Physics Publishing
Collection year 2015
Language eng
Formatted abstract
Freestanding membranes created from Bombyx mori silk fibroin (BMSF) offer a potential vehicle for corneal cell transplantation since they are transparent and support the growth of human corneal epithelial (HCE) cells. Fibroin derived from the wild silkworm Antheraea pernyi (APSF) might provide a superior material by virtue of containing putative cell-attachment sites that are absent from BMSF. Thus we have investigated the feasibility of producing transparent, freestanding membranes from APSF and have analysed the behaviour of HCE cells on this material. No significant differences in cell numbers or phenotype were observed in short term HCE cell cultures established on either fibroin. Production of transparent freestanding APSF membranes, however, proved to be problematic as cast solutions of APSF were more prone to becoming opaque, displayed significantly lower permeability and were more brittle than BMSF-membranes. Cultures of HCE cells established on either membrane developed a normal stratified morphology with cytokeratin pair 3/12 being immuno-localized to the superficial layers. We conclude that while it is feasible to produce transparent freestanding membranes from APSF, the technical difficulties associated with this biomaterial, along with an absence of enhanced cell growth, currently favour the continued development of BMSF as a preferred vehicle for corneal cell transplantation. Nevertheless, it remains possible that refinement of techniques for processing APSF might yet lead to improvements in the handling properties and performance of this material.
Keyword Corneal epithelium
Corneal transplantation
Silk fibroin
Bombyx mori
Antheraea pernyi
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article no.: 025016

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Medicine Publications
Australian Institute for Bioengineering and Nanotechnology Publications
 
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