Phylogenetic diversity of bacterial communities in bovine rumen as affected by diets and microenvironments

Kim, Minseok, Morrison, Mark and Yu, Zhongtang (2011) Phylogenetic diversity of bacterial communities in bovine rumen as affected by diets and microenvironments. Folia Microbiologica, 56 5: 453-458. doi:10.1007/s12223-011-0066-5


Author Kim, Minseok
Morrison, Mark
Yu, Zhongtang
Title Phylogenetic diversity of bacterial communities in bovine rumen as affected by diets and microenvironments
Journal name Folia Microbiologica   Check publisher's open access policy
ISSN 0015-5632
1874-9356
Publication date 2011
Sub-type Article (original research)
DOI 10.1007/s12223-011-0066-5
Open Access Status
Volume 56
Issue 5
Start page 453
End page 458
Total pages 6
Place of publication Dordrecht, Netherlands
Publisher Springer
Language eng
Abstract Phylogenetic analysis was conducted to examine ruminal bacteria in two ruminal fractions (adherent fraction vs. liquid fraction) collected from cattle fed with two different diets: forage alone vs. forage plus concentrate. One hundred forty-four 16S rRNA gene (rrs) sequences were obtained from clone libraries constructed from the four samples. These rrs sequences were assigned to 116 different operational taxonomic units (OTUs) defined at 0. 03 phylogenetic distance. Most of these OTUs could not be assigned to any known genus. The phylum Firmicutes was represented by approximately 70% of all the sequences. By comparing to the OTUs already documented in the rumen, 52 new OTUs were identified. UniFrac, SONS, and denaturing gradient gel electrophoresis analyses revealed difference in diversity between the two fractions and between the two diets. This study showed that rrs sequences recovered from small clone libraries can still help identify novel species-level OTUs.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Diamantina Institute Publications
 
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