Identification of metabolically active proteobacterial and archaeal communities in the rumen by DNA- and RNA-derived 16S rRNA gene

Kang S.H., Evans P., Morrison M. and Mcsweeney C. (2013) Identification of metabolically active proteobacterial and archaeal communities in the rumen by DNA- and RNA-derived 16S rRNA gene. Journal of Applied Microbiology, 115 3: 644-653. doi:10.1111/jam.12270


Author Kang S.H.
Evans P.
Morrison M.
Mcsweeney C.
Title Identification of metabolically active proteobacterial and archaeal communities in the rumen by DNA- and RNA-derived 16S rRNA gene
Journal name Journal of Applied Microbiology   Check publisher's open access policy
ISSN 1364-5072
1365-2672
Publication date 2013-09
Year available 2013
Sub-type Article (original research)
DOI 10.1111/jam.12270
Open Access Status
Volume 115
Issue 3
Start page 644
End page 653
Total pages 10
Place of publication West Sussex United Kingdom
Publisher Wiley-Blackwell Publishing
Collection year 2014
Language eng
Formatted abstract
Aims: To gain new insights into the metabolic contribution of bacterial group in the rumen.

Methods and Results: Both DNA- and RNA-derived bacterial 16S ribosomal materials from bovine rumen contents were used as the template for bacterial community and analyse microbiota by three methods namely custom phylogenetic microarray, quantitative real-time PCR and denaturing gradient gel electrophoresis techniques. Bacterial analysis showed that genera affiliating with the Proteobacteria apparently made a greater metabolic contribution to rumen function than their population sizes indicated. Analysis of another rumen microbial group, the methanogens, using clone libraries for the expressed methyl coenzyme reductase subunit A (mcrA) revealed that an uncultivated methanogen clade contributes one-third of RNA-derived mcrA sequences based on a limited number of clones analysed. These uncultivated methanogen species were not observed in the mcrA gene library based on the DNA-derived sequences.

Conclusions: The comparison of results obtained from DNA- and RNA-derived materials suggests that some of the Proteobacteria and novel methanogen species appeared to be low in abundance in the rumen maintained on grain-based diets might play a greater role in rumen metabolism.

Significance and Impact of the Study: These studies provide the first report to compare high-throughput analysis of bacterial 16S rRNA genes from DNA- and RNA-derived materials to indicate differences that species make to community structure and metabolic activity.
Keyword 16S rRNA gene
McrA
Metabolic activity
Methanogen
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
UQ Diamantina Institute Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 12 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 12 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Mon, 17 Mar 2014, 09:08:55 EST by System User on behalf of UQ Diamantina Institute