Genomic differences between Fibrobacter succinogenes S85 and Fibrobacter intestinalis DR7, identified by suppression subtractive hybridization

Qi, M., Nelson, K. E., Daugherty, S. C., Nelson, W. C., Hance, I. R., Morrison, M. and Forsberg, C. W. (2008) Genomic differences between Fibrobacter succinogenes S85 and Fibrobacter intestinalis DR7, identified by suppression subtractive hybridization. Applied and Environmental Microbiology, 74 4: 987-993. doi:10.1128/AEM.02514-07

Attached Files (Some files may be inaccessible until you login with your UQ eSpace credentials)
Name Description MIMEType Size Downloads
UQ325735_OA.pdf Full text (open access) application/pdf 194.14KB 0

Author Qi, M.
Nelson, K. E.
Daugherty, S. C.
Nelson, W. C.
Hance, I. R.
Morrison, M.
Forsberg, C. W.
Title Genomic differences between Fibrobacter succinogenes S85 and Fibrobacter intestinalis DR7, identified by suppression subtractive hybridization
Journal name Applied and Environmental Microbiology   Check publisher's open access policy
ISSN 0099-2240
1098-5336
Publication date 2008-01-01
Year available 2008
Sub-type Article (original research)
DOI 10.1128/AEM.02514-07
Open Access Status File (Publisher version)
Volume 74
Issue 4
Start page 987
End page 993
Total pages 7
Place of publication Washington, DC United States
Publisher American Society for Microbiology
Collection year 2009
Language eng
Subject 1305 Biotechnology
2300 Environmental Science
2404 Microbiology
Abstract Fibrobacter is a highly cellulolytic genus commonly found in the rumen of ruminant animals and cecum of monogastric animals. In this study, suppression subtractive hybridization was used to identify the genes present in Fibrobacter succinogenes S85 but absent from F. intestinalis DR7. A total of 1,082 subtractive clones were picked, plasmids were purified, and inserts were sequenced, and the clones lacking homology to F. intestinalis were confirmed by Southern hybridization. By comparison of the sequences of the clones to one another and to those of the F. succinogenes genome, 802 sequences or 955 putative genes, comprising approximately 409 kb of F. succinogenes genomic DNA, were identified that lack similarity to those of F. intestinalis chromosomal DNA. The functional groups of genes, including those involved in cell envelope structure and function, energy metabolism, and transport and binding, had the largest number of genes specific to F. succinogenes. Low-stringency Southern hybridization showed that at least 37 glycoside hydrolases are shared by both species. A cluster of genes responsible for heme, porphyrin, and cobalamin biosynthesis in F. succinogenes S85 was either missing from or not functional in F. intestinalis DR7, which explains the requirement of vitamin B12 for the growth of the F. intestinalis species. Two gene clusters encoding NADH-ubiquinone oxidoreductase subunits probably shared by Fibrobacter genera appear to have an important role in energy metabolism. Copyright
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Supplemental material (read only) - http://aem.asm.org/content/74/4/987/suppl/DC1

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Diamantina Institute Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 5 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 6 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Mon, 17 Mar 2014, 19:07:32 EST by System User on behalf of UQ Diamantina Institute