RNF14 is a regulator of mitochondrial and immune function in muscle

Ingham, Aaron B., Osborne, Simone A., Menzies, Moira, Briscoe, Suzie, Chen, Wei, Kongsuwan, Kritaya, Reverter, Antonio, Jeanes, Angela, Dalrymple, Brian P., Wijffels, Gene, Seymour, Robert and Hudson, Nicholas J. (2014) RNF14 is a regulator of mitochondrial and immune function in muscle. BMC Systems Biology, 8 1: 1-12. doi:10.1186/1752-0509-8-10


Author Ingham, Aaron B.
Osborne, Simone A.
Menzies, Moira
Briscoe, Suzie
Chen, Wei
Kongsuwan, Kritaya
Reverter, Antonio
Jeanes, Angela
Dalrymple, Brian P.
Wijffels, Gene
Seymour, Robert
Hudson, Nicholas J.
Title RNF14 is a regulator of mitochondrial and immune function in muscle
Journal name BMC Systems Biology   Check publisher's open access policy
ISSN 1752-0509
Publication date 2014-01-29
Year available 2014
Sub-type Article (original research)
DOI 10.1186/1752-0509-8-10
Open Access Status DOI
Volume 8
Issue 1
Start page 1
End page 12
Total pages 12
Place of publication London, United Kingdom
Publisher BioMed Central
Collection year 2015
Language eng
Formatted abstract
Background: Muscle development and remodelling, mitochondrial physiology and inflammation are thought to be inter-related and to have implications for metabolism in both health and disease. However, our understanding of their molecular control is incomplete.
Results: In this study we have confirmed that the ring finger 14 protein (RNF14), a poorly understood transcriptional regulator, influences the expression of both mitochondrial and immune-related genes. The prediction was based on a combination of network connectivity and differential connectivity in cattle (a non-model organism) and mice data sets, with a focus on skeletal muscle. They assigned similar probability to mammalian RNF14 playing a regulatory role in mitochondrial and immune gene expression. To try and resolve this apparent ambiguity we performed a genome-wide microarray expression analysis on mouse C2C12 myoblasts transiently transfected with two Rnf14 transcript variants that encode 2 naturally occurring but different RNF14 protein isoforms. The effect of both constructs was significantly different to the control samples (untransfected cells and cells transfected with an empty vector). Cluster analyses revealed that transfection with the two Rnf14 constructs yielded discrete expression signatures from each other, but in both cases a substantial set of genes annotated as encoding proteins related to immune function were perturbed. These included cytokines and interferon regulatory factors. Additionally, transfection of the longer transcript variant 1 coordinately increased the expression of 12 (of the total 13) mitochondrial proteins encoded by the mitochondrial genome, 3 of which were significant in isolated pair-wise comparisons (Mt-coxII, Mt-nd2 and mt-nd4l). This apparent additional mitochondrial function may be attributable to the RWD protein domain that is present only in the longer RNF14 isoform.
Conclusions: RNF14 influences the expression of both mitochondrial and immune related genes in a skeletal muscle context, and has likely implications for the inter-relationship between bioenergetic status and inflammation.
Keyword Inflammation
Mitochondria
Muscle
Networks
RNF14
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article # 10

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Biomedical Sciences Publications
 
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