Nanoparticle imaging and diagnostic of Caenorhabditis elegans intracellular pH

Mathew, Neal D., Mathew, Mark D. and Surawski, Peter P.T. (2014) Nanoparticle imaging and diagnostic of Caenorhabditis elegans intracellular pH. Analytical Biochemistry, 450 1: 52-56. doi:10.1016/j.ab.2014.01.011


Author Mathew, Neal D.
Mathew, Mark D.
Surawski, Peter P.T.
Title Nanoparticle imaging and diagnostic of Caenorhabditis elegans intracellular pH
Formatted title
 Nanoparticle imaging and diagnostic of Caenorhabditis elegans intracellular pH
Journal name Analytical Biochemistry   Check publisher's open access policy
ISSN 0003-2697
1096-0309
Publication date 2014-04-01
Year available 2014
Sub-type Article (original research)
DOI 10.1016/j.ab.2014.01.011
Open Access Status
Volume 450
Issue 1
Start page 52
End page 56
Total pages 5
Place of publication Philadelphia, PA, United States
Publisher Elsevier
Collection year 2015
Language eng
Formatted abstract
A novel diagnostic tool has been developed for the characterization of intracellular pH (pHi) in the model organism Caenorhabditis elegans. This tool exploits the chemical stability of colloidal silica and the pH sensitivity of certain fluorescent dyes. Once ingested, the fluorescent colloidal dispersion yields a reliable visual indication of pH without the use of chemical fixatives or damaging the nematode. The pH-sensitive silica nanoparticles were visualized by confocal microscopy, and the fluorescence spectra from the internally referenced colloidal particulates were measured. By comparing the fluorescence profile of colloids in wild-type (N2) and mutant (eat-3) C. elegans against a calibration series, the intestinal pHi could be established in each population. The rapid characterization of pHi using this inexpensive nonintrusive technique has significant implications for disease research where C. elegans is used as a model organism.
Keyword Caenorhabditis elegans
Colloid
Core-shell
Intracellular pH (pHi)
Nanodiagnostic
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Biological Sciences Publications
 
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