Alternating current electrohydrodynamics induced nanoshearing and fluid micromixing for specific capture of cancer cells

Vaidyanathan, Ramanathan, Rauf, Sakandar, Dray, Eloïse, Shiddiky, Muhammad J. A. and Trau, Matt (2014) Alternating current electrohydrodynamics induced nanoshearing and fluid micromixing for specific capture of cancer cells. Chemistry: A European Journal, 20 13: 3724-3729. doi:10.1002/chem.201304590


Author Vaidyanathan, Ramanathan
Rauf, Sakandar
Dray, Eloïse
Shiddiky, Muhammad J. A.
Trau, Matt
Title Alternating current electrohydrodynamics induced nanoshearing and fluid micromixing for specific capture of cancer cells
Journal name Chemistry: A European Journal   Check publisher's open access policy
ISSN 0947-6539
1521-3765
Publication date 2014-02-23
Sub-type Article (original research)
DOI 10.1002/chem.201304590
Open Access Status
Volume 20
Issue 13
Start page 3724
End page 3729
Total pages 6
Place of publication Weinheim, Germany
Publisher Wiley
Collection year 2015
Language eng
Abstract within a few nanometers of an electrode surface. This force can be externally tuned via manipulating the applied ac-EHD field strength. The ability to manipulate ac-EHD induced forces and concomitant fluid micromixing can enhance fluid transport within the capture domain of the channel (e.g., transport of analytes and hence increase target-sensor interactions). This also provides a new capability to preferentially select strongly bound analytes over nonspecifically bound cells and molecules. To demonstrate the utility and versatility of nanoshearing phenomenon to specifically capture cancer cells, we present proof-of-concept data in lysed blood using two microfluidic devices containing a long array of asymmetric planar electrode pairs. Under the optimal experimental conditions, we achieved high capture efficiency (e.g., approximately 90%; %RSD=2, n=3) with a 10-fold reduction in nonspecific adsorption of non-target cells for the detection of whole cells expressing Human Epidermal Growth Factor Receptor 2 (HER2). We believe that our ac-EHD devices and the use of tuneable nanoshearing phenomenon may find relevance in a wide variety of biological and medical applications.
Keyword Cell isolation
Electrohydrodynamics
Fluid shear forces
Microfluidics
Nonspecific adsorption
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Fri, 07 Mar 2014, 13:35:07 EST by Ramanathan Pudhukode Vaidyanathan on behalf of Aust Institute for Bioengineering & Nanotechnology