Mutational analysis of two highly conserved motifs in the silencing suppressor encoded by tomato spotted wilt virus (genus Tospovirus, family Bunyaviridae)

Zhai, Ying, Bag, Sudeep, Mitter, Neena, Turina, Massimo and Pappu, Hanu R. (2013) Mutational analysis of two highly conserved motifs in the silencing suppressor encoded by tomato spotted wilt virus (genus Tospovirus, family Bunyaviridae). Archives of Virology, 159 6: 1499-1504. doi:10.1007/s00705-013-1928-8


Author Zhai, Ying
Bag, Sudeep
Mitter, Neena
Turina, Massimo
Pappu, Hanu R.
Title Mutational analysis of two highly conserved motifs in the silencing suppressor encoded by tomato spotted wilt virus (genus Tospovirus, family Bunyaviridae)
Formatted title
Mutational analysis of two highly conserved motifs in the silencing suppressor encoded by tomato spotted wilt virus (genus
Tospovirus, family Bunyaviridae)
Journal name Archives of Virology   Check publisher's open access policy
ISSN 0304-8608
1432-8798
Publication date 2013-12-22
Year available 2013
Sub-type Article (original research)
DOI 10.1007/s00705-013-1928-8
Open Access Status
Volume 159
Issue 6
Start page 1499
End page 1504
Total pages 6
Place of publication Vienna, Austria
Publisher Springer Wien
Collection year 2014
Language eng
Formatted abstract
Tospoviruses cause serious economic losses to a wide range of field and horticultural crops on a global scale. The NSs gene encoded by tospoviruses acts as a suppressor of host plant defense. We identified amino acid motifs that are conserved in all of the NSs proteins of tospoviruses for which the sequence is known. Using tomato spotted wilt virus (TSWV) as a model, the role of these motifs in suppressor activity of NSs was investigated. Using site-directed point mutations in two conserved motifs, glycine, lysine and valine/threonine (GKV/T) at positions 181-183 and tyrosine and leucine (YL) at positions 412-413, and an assay to measure the reversal of gene silencing in Nicotiana benthamiana line 16c, we show that substitutions (K182 to A, and L413 to A) in these motifs abolished suppressor activity of the NSs protein, indicating that these two motifs are essential for the RNAi suppressor function of tospoviruses.
Keyword Tomato spotted wilt virus
Silencing suppressor
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online: 22 December 2013.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Alliance for Agriculture and Food Innovation
Official 2014 Collection
 
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Citation counts: TR Web of Science Citation Count  Cited 8 times in Thomson Reuters Web of Science Article | Citations
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Created: Fri, 07 Mar 2014, 13:28:24 EST by Dr Neena Mitter on behalf of Centre for Plant Science