The plant-derived decapeptide OSIP108 interferes with Candida albicans biofilm formation without affecting cell viability

Delattin, Nicolas, De Brucker, Katrijn, Craik, David J., Cheneval, Olivier, Frohlich, Mirjam, Veber, Matija, Girandon, Lenart, Davis, Tayla, R., Weeks, Anne E., Kumamoto, Carol A., Cos, Paul, Coenye, Tom, De Coninck, Barbara, Cammue, Bruno P. A. and Thevissen, Karin (2014) The plant-derived decapeptide OSIP108 interferes with Candida albicans biofilm formation without affecting cell viability. Antimicrobial Agents and Chemotherapy, 58 5: 2647-2656. doi:10.1128/AAC.01274-13

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Author Delattin, Nicolas
De Brucker, Katrijn
Craik, David J.
Cheneval, Olivier
Frohlich, Mirjam
Veber, Matija
Girandon, Lenart
Davis, Tayla, R.
Weeks, Anne E.
Kumamoto, Carol A.
Cos, Paul
Coenye, Tom
De Coninck, Barbara
Cammue, Bruno P. A.
Thevissen, Karin
Title The plant-derived decapeptide OSIP108 interferes with Candida albicans biofilm formation without affecting cell viability
Formatted title
The plant-derived decapeptide OSIP108 interferes with Candida albicans biofilm formation without affecting cell viability
Journal name Antimicrobial Agents and Chemotherapy   Check publisher's open access policy
ISSN 0066-4804
1098-6596
Publication date 2014-02-24
Sub-type Article (original research)
DOI 10.1128/AAC.01274-13
Open Access Status File (Publisher version)
Volume 58
Issue 5
Start page 2647
End page 2656
Total pages 10
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Collection year 2015
Language eng
Formatted abstract
We previously identified a decapeptide from the model plant Arabidopsis thaliana, OSIP108, which is induced upon fungal pathogen infection. In this study, we demonstrated that OSIP108 interferes with biofilm formation of the fungal pathogen C. albicans, without affecting viability or growth of C. albicans cells. OSIP108 displayed no cytotoxicity against various human cell lines. Furthermore, OSIP108 enhanced the activity of the antifungal agents amphotericin B and caspofungin in vitro and in vivo in a Caenorhabditis elegans-C. albicans biofilm infection model. These data point to the potential use of OSIP108 in combination therapy with conventional antifungal agents. In a first attempt to unravel its mode of action, we screened a library of 137 homozygous C. albicans mutants, affected in genes encoding for cell wall proteins or transcription factors important for biofilm formation, for altered OSIP108-sensitivity. We identified 9 OSIP108-tolerant C. albicans mutants, either defective in components important for cell wall integrity or yeast-to-hypha transition. In line with these findings, we demonstrated that OSIP108 activates the C. albicans cell wall integrity pathway and that its antibiofilm activity can be blocked by compounds inhibiting the yeast-to-hypha transition. Furthermore, we found that OSIP108 is predominantly localized at the C. albicans cell surface. These data point to interference of OSIP108 with cell wall-related processes of C. albicans, resulting in impaired biofilm formation.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
Institute for Molecular Bioscience - Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 12 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 13 times in Scopus Article | Citations
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Created: Wed, 05 Mar 2014, 11:03:39 EST by Susan Allen on behalf of Institute for Molecular Bioscience