Combining chemical analysis and bioanalytical tools for a comprehensive assessment of organic compounds in recycled water

Tang, Janet, Busetti, Francesco, Charrois, Jeffrey and Escher, Beate (2013). Combining chemical analysis and bioanalytical tools for a comprehensive assessment of organic compounds in recycled water. In: 3rd SETAC Australasia Conference: Melbourne 2013. Conference Handbook. 3rd SETAC Australasia Conference: Melbourne 2013, Melbourne, VIC, Australia, (). 1-3 October, 2013.

Author Tang, Janet
Busetti, Francesco
Charrois, Jeffrey
Escher, Beate
Title of paper Combining chemical analysis and bioanalytical tools for a comprehensive assessment of organic compounds in recycled water
Conference name 3rd SETAC Australasia Conference: Melbourne 2013
Conference location Melbourne, VIC, Australia
Conference dates 1-3 October, 2013
Convener Society of Environmental Toxicology and Chemistry (SETAC)
Proceedings title 3rd SETAC Australasia Conference: Melbourne 2013. Conference Handbook
Place of Publication Melbourne, VIC, Australia
Publisher SETAC Australasia
Publication Year 2013
Sub-type Published abstract
Language eng
Formatted Abstract/Summary
Compliance monitoring of drinking water and recycled water in Australia is predominantly based on chemical assessments using instrumental analysis. Bioanalytical tools have the potential to assess the mixture effects according to the mode of toxic action and can complement chemical analytical monitoring. In this study, grab water samples were collected from an Australian Wastewater Treatment Plant (WWTP) with secondary treatment process including activated sludge treatment and an Advanced Water Recycling Plant (AWRP). As a trial a small portion of the secondary wastewater has been pumped to the AWRP. At the AWRP, water is treated through ultrafiltration, chloramination, reverse osmosis (RO) and UV disinfection. Analysis of 278 compounds including pesticides, herbicides, pharmaceuticals, endocrine disrupting compounds and X-ray contrast media was undertaken in different points along the treatment train. Treatment efficiently removed organic compounds. Only very low levels of the anticorrosive compound tolyltriazole, the plasticizer bisphenol A, the pharmaceutical triclosan and the pesticides MCPA and 3,4-dichloroaniline were detected in the post-RO water. The positive low-level detections of these compounds in post-RO water were found to be consistent with previous monitoring programs except for the pesticides MCPA and 3,4-dichloraniline, which were not detected before. Complete removal of all compounds targeted was observed in the post-UV water. In parallel to the chemical screening, a battery of cell-based bioassays covering a wide range of modes of toxic action was used to evaluate the samples. The identified chemicals were mixed in the concentration ratios detected and underwent bioanalytical assessment. The effects caused by these designed mixtures were compared to the effects of the corresponding entire samples. For receptor-mediated biological endpoints such as photosynthesis inhibition, where a small number of well-defined chemicals are known to be active, the majority of the effect could be explained by the identified chemicals. For non-specific bioassays such as cytotoxicity or oxidative stress response, where all or many compounds contribute to the mixture effects, the detected chemicals could explain less than 1% of the measured effect, meaning that non-target chemicals and transformation products contribute to the mixture effects. Nevertheless the levels of organic compounds and effects are of no concern post-RO as was demonstrated by comparison with the Australian Guidelines for Water Recycling (AGWR). We translated the established chemical guideline values into tentative effect-based trigger values (EBT) and none of the recycled water samples exceeded these thresholds.
Q-Index Code EX
Q-Index Status Provisional Code

Document type: Conference Paper
Collection: National Research Centre for Environmental Toxicology Publications
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Created: Tue, 04 Mar 2014, 17:07:03 EST by Ms Janet Tang on behalf of National Res Centre For Environmental Toxicology