A novel method for sampling bacteria on plant root and soil surfaces at the microhabitat scale

Dennis, Paul G., Miller, Anthony J., Clark, Ian M., Taylor, Richard G., Valsami-Jones, Eugenia and Hirsch, Penny R. (2008) A novel method for sampling bacteria on plant root and soil surfaces at the microhabitat scale. Journal of Microbiological Methods, 75 1: 12-18. doi:10.1016/j.mimet.2008.04.013

Author Dennis, Paul G.
Miller, Anthony J.
Clark, Ian M.
Taylor, Richard G.
Valsami-Jones, Eugenia
Hirsch, Penny R.
Title A novel method for sampling bacteria on plant root and soil surfaces at the microhabitat scale
Journal name Journal of Microbiological Methods   Check publisher's open access policy
ISSN 0167-7012
Publication date 2008
Year available 2008
Sub-type Article (original research)
DOI 10.1016/j.mimet.2008.04.013
Volume 75
Issue 1
Start page 12
End page 18
Total pages 7
Place of publication Amsterdam, Netherlands
Publisher Elsevier BV
Collection year 2009
Language eng
Subject 1305 Biotechnology
2402 Applied Microbiology and Biotechnology
2404 Microbiology
Abstract This study reports the first method for sampling bacteria at a spatial scale approximating a microhabitat. At the core of this method is the use of tungsten rods with laser-cut tips of known surface area (0.013 mm 2). Exposed plant root or soil surfaces were viewed with a dissecting microscope and micro-sampling rods were guided to sample sites using a micro-manipulator. Bacteria that adhered to the sampling tips were then recovered for microbiological analyses. The efficiency of this method for removing bacteria from root surfaces was similar to that with which bacteria are recovered from dissected root segments using the conventional technique of washing. However, as the surface area of the micro-sampling tips was known, the new method has the advantage of eliminating inaccuracy in estimates of bacterial densities due to inaccurate estimation of the root or soil surface sampled. When used to investigate spatial distributions of rhizoplane bacteria, the new technique revealed trends that were consistent with those reported with existing methods, while providing access to additional information about community structure at a much smaller spatial scale. The spatial scale of this new method is ca. 1000-times smaller than other sampling methods involving swabbing. This novel technique represents an important methodological step facilitating microbial ecological investigations at a microhabitat scale. Crown Copyright
Keyword Micro-sampling
Rhizosphere bacteria
Soil bacteria
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Agriculture and Food Sciences
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Created: Tue, 04 Mar 2014, 15:47:39 EST by Paul Dennis on behalf of School of Agriculture and Food Sciences