Non-destructive micro-scale sampling of bacteria from a root surface using a tungsten rod with a standardised sampling area controlled by a micromanipulator

Dennis, Paul, Miller, Anthony and Hirsch, Penny (2006). Non-destructive micro-scale sampling of bacteria from a root surface using a tungsten rod with a standardised sampling area controlled by a micromanipulator. In Peter Schweiger and Roger Finlay (Ed.), Handbook of methods used in rhizosphere research (pp. 384-385) Birmensdorf, Switzerland: Swiss Federal Research Institute WSL.

Author Dennis, Paul
Miller, Anthony
Hirsch, Penny
Title of chapter Non-destructive micro-scale sampling of bacteria from a root surface using a tungsten rod with a standardised sampling area controlled by a micromanipulator
Title of book Handbook of methods used in rhizosphere research
Place of Publication Birmensdorf, Switzerland
Publisher Swiss Federal Research Institute WSL
Publication Year 2006
Sub-type Research book chapter (original research)
Series COST
ISBN 3905621355
9783905621358
Editor Peter Schweiger
Roger Finlay
Volume number 631
Chapter number 4.1
Start page 384
End page 385
Total pages 2
Total chapters 5
Language eng
Formatted Abstract/Summary
Concept: A surface such as the rhizoplane is touched with the tip of a sampling rod to collect bacteria, which may be recovered for culture/culture-independent microbiological analyses. The method is non-destructive and allows repeated measurements from the same plants over time. By laser cutting the rods a tip of standardised surface area is achieved which enables expressing bacterial samples per unit area. The scale of the sampling tip is designed to allow separate measurements within different zones on the root with contrasting exudation patterns. In principle, this sampling technique could be applied to any surface where micro-scale interactions are of interest, and will allow the detailed dynamic mapping of key rhizosphere processes such as nutrient cycling and pathogen responses...
Q-Index Code B1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Book Chapter
Collection: School of Agriculture and Food Sciences
 
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Created: Tue, 04 Mar 2014, 15:37:00 EST by Paul Dennis on behalf of School of Agriculture and Food Sciences