NanoCAGE analysis of the mouse olfactory epithelium identifies the expression of vomeronasal receptors and of proximal LINE elements

Pascarella, Giovanni, Lazarevic, Dejan, Plessy, Charles, Bertin, Nicolas, Akalin, Altuna, Vlachouli, Christina, Simone, Roberto, Faulkner, Geoffry J., Zucchelli, Silvia, Kawai, Jun, Daub, Carsten O., Hayashizaki, Yoshihide, Lenhard, Boris, Carninci, Piero and Gustincich, Stefano (2014) NanoCAGE analysis of the mouse olfactory epithelium identifies the expression of vomeronasal receptors and of proximal LINE elements. Frontiers in Cellular Neuroscience, 8 FEB: . doi:10.3389/fncel.2014.00041

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Author Pascarella, Giovanni
Lazarevic, Dejan
Plessy, Charles
Bertin, Nicolas
Akalin, Altuna
Vlachouli, Christina
Simone, Roberto
Faulkner, Geoffry J.
Zucchelli, Silvia
Kawai, Jun
Daub, Carsten O.
Hayashizaki, Yoshihide
Lenhard, Boris
Carninci, Piero
Gustincich, Stefano
Title NanoCAGE analysis of the mouse olfactory epithelium identifies the expression of vomeronasal receptors and of proximal LINE elements
Journal name Frontiers in Cellular Neuroscience   Check publisher's open access policy
ISSN 1662-5102
Publication date 2014-02-18
Year available 2014
Sub-type Article (original research)
DOI 10.3389/fncel.2014.00041
Open Access Status DOI
Volume 8
Issue FEB
Total pages 10
Place of publication Lausanne, Switzerland
Publisher Frontiers Research Foundation
Collection year 2015
Language eng
Formatted abstract
By coupling laser capture microdissection to nanoCAGE technology and next-generation sequencing we have identified the genome-wide collection of active promoters in the mouse Main Olfactory Epithelium (MOE). Transcription start sites (TSSs) for the large majority of Olfactory Receptors (ORs) have been previously mapped increasing our understanding of their promoter architecture. Here we show that in our nanoCAGE libraries of the mouse MOE we detect a large number of tags mapped in loci hosting Type-1 and Type-2 Vomeronasal Receptors genes (V1Rs and V2Rs). These loci also show a massive expression of Long Interspersed Nuclear Elements (LINEs). We have validated the expression of selected receptors detected by nanoCAGE with in situ hybridization, RT-PCR and qRT-PCR. This work extends the repertory of receptors capable of sensing chemical signals in the MOE, suggesting intriguing interplays between MOE and VNO for pheromone processing and positioning transcribed LINEs as candidate regulatory RNAs for VRs expression.
Keyword Main olfactory epithelium
MOE
V1Rs
V2Rs
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article number 41.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Biomedical Sciences Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 8 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 9 times in Scopus Article | Citations
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