Mapping Organelle Motion Reveals a Vesicular Conveyor Belt Spatially Replenishing Secretory Vesicles in Stimulated Chromaffin Cells

Maucort, Guillaume, Kasula, Ravikiran, Papadopulos, Andreas, Nieminen, Timo A., Rubinsztein-Dunlop, Halina and Meunier, Frederic A. (2014) Mapping Organelle Motion Reveals a Vesicular Conveyor Belt Spatially Replenishing Secretory Vesicles in Stimulated Chromaffin Cells. PLoS One, 9 1: . doi:10.1371/journal.pone.0087242


Author Maucort, Guillaume
Kasula, Ravikiran
Papadopulos, Andreas
Nieminen, Timo A.
Rubinsztein-Dunlop, Halina
Meunier, Frederic A.
Title Mapping Organelle Motion Reveals a Vesicular Conveyor Belt Spatially Replenishing Secretory Vesicles in Stimulated Chromaffin Cells
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2014-01
Year available 2014
Sub-type Article (original research)
DOI 10.1371/journal.pone.0087242
Open Access Status DOI
Volume 9
Issue 1
Total pages 9
Place of publication San Francisco, CA United States
Publisher Public Library of Science
Collection year 2015
Language eng
Formatted abstract
 How neurosecretory cells spatially adjust their secretory vesicle pools to replenish those that have fused and released their hormonal content is currently unknown. Here we designed a novel set of image analyses to map the probability of tracked organelles undergoing a specific type of movement (free, caged or directed). We then applied our analysis to time-lapse z-stack confocal imaging of secretory vesicles from bovine Chromaffin cells to map the global changes in vesicle motion and directionality occurring upon secretagogue stimulation. We report a defined region abutting the cortical actin network that actively transports secretory vesicles and is dissipated by actin and microtubule depolymerizing drugs. The directionality of this “conveyor belt” towards the cell surface is activated by stimulation. Actin and microtubule networks therefore cooperatively probe the microenvironment to transport secretory vesicles to the periphery, providing a mechanism whereby cells globally adjust their vesicle pools in response to secretagogue stimulation.
Keyword Plasma Membrane
Actin
Exocytosis
Granules
Endocytosis
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Mathematics and Physics
Queensland Brain Institute Publications
Official 2015 Collection
 
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Citation counts: TR Web of Science Citation Count  Cited 4 times in Thomson Reuters Web of Science Article | Citations
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