Antiviral CD8 T cells recognize Borna disease virus antigen transgenically expressed in either neurons or astrocytes

Baur, Karen, Rauer, Mathias, Richter, Kirsten, Pagenstecher, Axel, Gotz, Jurgen, Hausmann, Jurgen and Staeheli, Peter (2008) Antiviral CD8 T cells recognize Borna disease virus antigen transgenically expressed in either neurons or astrocytes. Journal of Virology, 82 6: 3099-3108. doi:10.1128/JVI.02479-07


Author Baur, Karen
Rauer, Mathias
Richter, Kirsten
Pagenstecher, Axel
Gotz, Jurgen
Hausmann, Jurgen
Staeheli, Peter
Title Antiviral CD8 T cells recognize Borna disease virus antigen transgenically expressed in either neurons or astrocytes
Journal name Journal of Virology   Check publisher's open access policy
ISSN 0022-538X
1098-5514
Publication date 2008-03-01
Year available 2008
Sub-type Article (original research)
DOI 10.1128/JVI.02479-07
Open Access Status DOI
Volume 82
Issue 6
Start page 3099
End page 3108
Total pages 10
Place of publication Washington, DC United States
Publisher American Society for Microbiology
Collection year 2009
Language eng
Subject 2403 Immunology
Abstract Borna disease virus (BDV) can persistently infect the central nervous system (CNS) of mice. The infection remains nonsymptomatic as long as antiviral CD8 T cells do not infiltrate the infected brain. BDY mainly infects neurons which reportedly carry few, if any, major histocompatibility complex class I molecules on the surface. Therefore, it remains unclear whether T cells can recognize replicating virus in these cells or whether cross-presentation of viral antigen by other cell types is important for immune recognition of BDV. To distinguish between these possibilities, we used two lines of transgenic mice that strongly express the N protein of BDV in either neurons (Neuro-N) or astrocytes (Astro-N). Since these animals are tolerant to the neo-self-antigen, we adoptively transferred T cells with specificity for BDV N. In nontransgenic mice persistently infected with BDV, the transferred cells accumulated in the brain parenchyma along with immune cells of host origin and efficiently induced neurological disease. Neurological disease was also observed if antiviral T cells were injected into the brains of Astro-N or Neuro-N but not nontransgenic control mice. Our results demonstrate that CD8 T cells can recognize foreign antigen on neurons and astrocytes even in the absence of infection or inflammation, indicating that these CNS cell types are playing an active role in immune recognition of viruses. Copyright
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Brain Institute Publications
 
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