The role of protein phosphatase 2A catalytic subunit Cα in embryogenesis: evidence from sequence analysis and localization studies

Goetz, Juergen and Kues, Wilfried (1999) The role of protein phosphatase 2A catalytic subunit Cα in embryogenesis: evidence from sequence analysis and localization studies. Biological Chemistry, 380 9: 1117-1120. doi:10.1515/BC.1999.139

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Author Goetz, Juergen
Kues, Wilfried
Title The role of protein phosphatase 2A catalytic subunit Cα in embryogenesis: evidence from sequence analysis and localization studies
Journal name Biological Chemistry   Check publisher's open access policy
ISSN 1431-6730
1437-4315
Publication date 1999-09
Year available 1999
Sub-type Article (original research)
DOI 10.1515/BC.1999.139
Open Access Status File (Publisher version)
Volume 380
Issue 9
Start page 1117
End page 1120
Total pages 4
Place of publication Berlin, Germany
Publisher Walter de Gruyter
Language eng
Abstract Protein phosphatase 2A (PP2A) constitutes one of the major families of protein serine/threonine phosphatases found in all eukaryotic cells. PP2A holoenzymes are composed of a catalytic subunit complexed with a structural regulatory subunit of 65 kDa. These core subunits associate with regulatory subunits of various sizes to form different heterotrimers which have been purified and evaluated with regard to substrate specificity. In fully differentiated tissues PP2A expression levels are highest in the brain, however, relatively little is known about expression in the developing embryo. In order to determine the composition of PP2A catalytic subunits in the mouse, cDNAs were cloned and the genomic organization of PP2A Cα was determined. By a gene targeting approach in the mouse, we have previously shown that the absence of the major catalytic subunit of PP2A, Cα, resulted in embryonic lethality around embryonic day E6.5. No mesoderm was formed which implied that PP2A plays a crucial role in gastrulation. Here, we extended our studies and analyzed wildtype embryos for Cα expression at subsequent stages of development. After gastrulation is completed, we find high expression of Cα restricted to the neural folds, which suggests that PP2A plays an additional pivotal role in neurulation.
Keyword Neural fold
Protein phosphatase 2A
Whole-mount immunohistochemistry
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
Queensland Brain Institute Publications
 
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