A simple, one-tube assay for the simultaneous detection and diagnosis of ten Australian poultry Eimeria

Godwin, Rosamond M. and Morgan, Jess A. T. (2013) A simple, one-tube assay for the simultaneous detection and diagnosis of ten Australian poultry Eimeria. Electrophoresis, 35 4: 494-502. doi:10.1002/elps.201300286


Author Godwin, Rosamond M.
Morgan, Jess A. T.
Title A simple, one-tube assay for the simultaneous detection and diagnosis of ten Australian poultry Eimeria
Formatted title
A simple, one-tube assay for the simultaneous detection and diagnosis of ten Australian poultry Eimeria
Journal name Electrophoresis   Check publisher's open access policy
ISSN 0173-0835
1522-2683
Publication date 2013
Sub-type Article (original research)
DOI 10.1002/elps.201300286
Open Access Status
Volume 35
Issue 4
Start page 494
End page 502
Total pages 9
Place of publication Weinheim, Germany
Publisher Wiley - V C H Verlag
Collection year 2014
Language eng
Formatted abstract
Coccidiosis is a costly worldwide enteric disease of chickens caused by parasites of the genus Eimeria. At present, there are seven described species that occur globally and a further three undescribed, operational taxonomic units (OTUs X, Y, and Z) that are known to infect chickens from Australia. Species of Eimeria have both overlapping morphology and pathology and frequently occur as mixed-species infections. This makes definitive diagnosis with currently available tests difficult and, to date, there is no test for the detection of the three OTUs. This paper describes the development of a PCR-based assay that is capable of detecting all ten species of Eimeria, including OTUs X, Y, and Z in field samples. The assay is based on a single set of generic primers that amplifies a single diagnostic fragment from the mitochondrial genome of each species. This one-tube assay is simple, low-cost, and has the capacity to be high throughput. It will therefore be of great benefit to the poultry industry for Eimeria detection and control, and the confirmation of identity and purity of vaccine strains. 
Keyword CE
Diagnostics
Eimeria
Mitochondrial DNA
PCR
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Alliance for Agriculture and Food Innovation
Official 2014 Collection
 
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Citation counts: TR Web of Science Citation Count  Cited 3 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 4 times in Scopus Article | Citations
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Created: Thu, 20 Feb 2014, 17:45:15 EST by Dr Jessica Morgan on behalf of School of Biomedical Sciences