Experimentally Infected Domestic Ducks Show Efficient Transmission of Indonesian H5N1 Highly Pathogenic Avian Influenza Virus, but Lack Persistent Viral Shedding

Wibawa, Hendra, Bingham, John, Nuradji, Harimurti, Lowther, Sue, Payne, Jean, Harper, Jenni, Junaidi, Akhmad, Middleton, Deborah and Meers, Joanne (2014) Experimentally Infected Domestic Ducks Show Efficient Transmission of Indonesian H5N1 Highly Pathogenic Avian Influenza Virus, but Lack Persistent Viral Shedding. PLoS One, 9 1: e83417.1-e83417.11. doi:10.1371/journal.pone.0083417


Author Wibawa, Hendra
Bingham, John
Nuradji, Harimurti
Lowther, Sue
Payne, Jean
Harper, Jenni
Junaidi, Akhmad
Middleton, Deborah
Meers, Joanne
Title Experimentally Infected Domestic Ducks Show Efficient Transmission of Indonesian H5N1 Highly Pathogenic Avian Influenza Virus, but Lack Persistent Viral Shedding
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2014-01
Year available 2014
Sub-type Article (original research)
DOI 10.1371/journal.pone.0083417
Open Access Status DOI
Volume 9
Issue 1
Start page e83417.1
End page e83417.11
Total pages 11
Place of publication San Francisco, CA United States
Publisher Public Library of Science
Collection year 2015
Language eng
Formatted abstract
 Ducks are important maintenance hosts for avian influenza, including H5N1 highly pathogenic avian influenza viruses. A previous study indicated that persistence of H5N1 viruses in ducks after the development of humoral immunity may drive viral evolution following immune selection. As H5N1 HPAI is endemic in Indonesia, this mechanism may be important in understanding H5N1 evolution in that region. To determine the capability of domestic ducks to maintain prolonged shedding of Indonesian clade 2.1 H5N1 virus, two groups of Pekin ducks were inoculated through the eyes, nostrils and oropharynx and viral shedding and transmission investigated. Inoculated ducks (n = 15), which were mostly asymptomatic, shed infectious virus from the oral route from 1 to 8 days post inoculation, and from the cloacal route from 2–8 dpi. Viral ribonucleic acid was detected from 1–15 days post inoculation from the oral route and 1–24 days post inoculation from the cloacal route (cycle threshold <40). Most ducks seroconverted in a range of serological tests by 15 days post inoculation. Virus was efficiently transmitted during acute infection (5 inoculation-infected to all 5 contact ducks). However, no evidence for transmission, as determined by seroconversion and viral shedding, was found between an inoculation-infected group (n = 10) and contact ducks (n = 9) when the two groups only had contact after 10 days post inoculation. Clinical disease was more frequent and more severe in contact-infected (2 of 5) than inoculation-infected ducks (1 of 15). We conclude that Indonesian clade 2.1 H5N1 highly pathogenic avian influenza virus does not persist in individual ducks after acute infection.
Keyword Anas Platyrhynchos
Asia
Evolution
Disease
Epidemiology
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Veterinary Science Publications
 
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