Inactivation of Pyruvate Kinase or the Phosphoenolpyruvate: Sugar Phosphotransferase System Increases Shikimic and Dehydroshikimic Acid Yields from Glucose in Bacillus subtilis

Licona-Cassani, Cuauthemoc, Lara, Alvaro R., Cabrera-Valladares, Natividad, Escalante, Adelfo, Hernandez-Chavez, Georgina, Martinez, Alfredo, Bolivar, Francisco and Gosset, Guillermo (2014) Inactivation of Pyruvate Kinase or the Phosphoenolpyruvate: Sugar Phosphotransferase System Increases Shikimic and Dehydroshikimic Acid Yields from Glucose in Bacillus subtilis. Journal of Molecular Microbiology and Biotechnology, 24 1: 37-45. doi:10.1159/000355264


Author Licona-Cassani, Cuauthemoc
Lara, Alvaro R.
Cabrera-Valladares, Natividad
Escalante, Adelfo
Hernandez-Chavez, Georgina
Martinez, Alfredo
Bolivar, Francisco
Gosset, Guillermo
Title Inactivation of Pyruvate Kinase or the Phosphoenolpyruvate: Sugar Phosphotransferase System Increases Shikimic and Dehydroshikimic Acid Yields from Glucose in Bacillus subtilis
Formatted title
Inactivation of Pyruvate Kinase or the Phosphoenolpyruvate: Sugar Phosphotransferase System Increases Shikimic and Dehydroshikimic Acid Yields from Glucose in Bacillus subtilis
Journal name Journal of Molecular Microbiology and Biotechnology   Check publisher's open access policy
ISSN 1464-1801
1660-2412
Publication date 2014-01
Year available 2013
Sub-type Article (original research)
DOI 10.1159/000355264
Open Access Status
Volume 24
Issue 1
Start page 37
End page 45
Total pages 9
Place of publication Basel, Switzerland
Publisher S. Karger AG
Collection year 2014
Language eng
Formatted abstract
The glycolytic intermediate phosphoenolpyruvate (PEP) is a precursor of several cellular components, including various aromatic compounds. Modifications to the PEP node such as PEP:sugar phosphotransferase system (PTS) or pyruvate kinase inactivation have been shown to have a positive effect on aromatics production capacity in Escherichia coli and Bacillus subtilis. In this study, pyruvate kinase and PTS-deficient B. subtilis strains were employed for the construction of derivatives lacking shikimate kinase activity that accumulate two industrially valuable chemicals, the intermediates of the common aromatic pathway, shikimic and dehydroshikimic acids. The pyruvate kinase-deficient strain (CLC6-PYKA) showed the best production parameters under resting-cell conditions. Compared to the PTS-deficient strain, the shikimic and dehydroshikimic acids specific production rates for CLC6-PYKA were 1.8- and 1.7-fold higher, respectively. A batch fermentor culture using complex media supplemented with 83 g/l of glucose was developed with strain CLC6-PYKA, where final titers of 4.67 g/l (shikimic acid) and 6.2 g/l (dehydroshikimic acid) were produced after 42 h.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 4 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 8 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Sun, 16 Feb 2014, 00:03:49 EST by System User on behalf of Aust Institute for Bioengineering & Nanotechnology