A new species of Burkholderia isolated from sugarcane roots promotes plant growth

Paungfoo-Lonhienne, Chanyarat, Lonhienne, Thierry G. A., Yeoh, Yun Kit, Webb, Richard I., Lakshmanan, Prakash, Chan, Cheong Xin, Lim, Phaik-Eem, Ragan, Mark A., Schmidt, Susanne and Hugenholtz, Philip (2014) A new species of Burkholderia isolated from sugarcane roots promotes plant growth. Microbial Biotechnology, 7 2: 142-154. doi:10.1111/1751-7915.12105


Author Paungfoo-Lonhienne, Chanyarat
Lonhienne, Thierry G. A.
Yeoh, Yun Kit
Webb, Richard I.
Lakshmanan, Prakash
Chan, Cheong Xin
Lim, Phaik-Eem
Ragan, Mark A.
Schmidt, Susanne
Hugenholtz, Philip
Title A new species of Burkholderia isolated from sugarcane roots promotes plant growth
Formatted title
A new species of Burkholderia isolated from sugarcane roots promotes plant growth
Journal name Microbial Biotechnology   Check publisher's open access policy
ISSN 1751-7915
Publication date 2014-03
Year available 2013
Sub-type Article (original research)
DOI 10.1111/1751-7915.12105
Open Access Status DOI
Volume 7
Issue 2
Start page 142
End page 154
Total pages 13
Place of publication Chichester, West Sussex, United Kingdom
Publisher Wiley-Blackwell Publishing
Collection year 2014
Language eng
Formatted abstract
Sugarcane is a globally important food, biofuel and biomaterials crop. High nitrogen (N) fertilizer rates aimed at increasing yield often result in environmental damage because of excess and inefficient application. Inoculation with diazotrophic bacteria is an attractive option for reducing N fertilizer needs. However, the efficacy of bacterial inoculants is variable, and their effective formulation remains a knowledge frontier. Here, we take a new approach to investigating diazotrophic bacteria associated with roots using culture-independent microbial community profiling of a commercial sugarcane variety (Q208A) in a field setting. We first identified bacteria that were markedly enriched in the rhizosphere to guide isolation and then tested putative diazotrophs for the ability to colonize axenic sugarcane plantlets (Q208A) and promote growth in suboptimal N supply. One isolate readily colonized roots, fixed N2 and stimulated growth of plantlets, and was classified as a new species, Burkholderia australis sp. nov. Draft genome sequencing of the isolate confirmed the presence of nitrogen fixation. We propose that culture-independent identification and isolation of bacteria that are enriched in rhizosphere and roots, followed by systematic testing and confirming their growth-promoting capacity, is a necessary step towards designing effective microbial inoculants.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article first published online: 19 December 2013.

 
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Created: Fri, 14 Feb 2014, 09:18:45 EST by Mrs Louise Nimwegen on behalf of School of Agriculture and Food Sciences