Antifungal activity of black tea polyphenols (catechins and theaflavins) against candida species

Sitheeque, M. A. M., Panagoda, G. J., Yau, J., Amarakoon, A. M. T., Udagam, U. R. N. and Samaranayake, L. P. (2009) Antifungal activity of black tea polyphenols (catechins and theaflavins) against candida species. Chemotherapy, 55 3: 189-196. doi:10.1159/000216836


Author Sitheeque, M. A. M.
Panagoda, G. J.
Yau, J.
Amarakoon, A. M. T.
Udagam, U. R. N.
Samaranayake, L. P.
Title Antifungal activity of black tea polyphenols (catechins and theaflavins) against candida species
Formatted title
Antifungal activity of black tea polyphenols (catechins and theaflavins) against Candida species 
Journal name Chemotherapy   Check publisher's open access policy
ISSN 0009-3157
1421-9794
Publication date 2009
Sub-type Article (original research)
DOI 10.1159/000216836
Open Access Status
Volume 55
Issue 3
Start page 189
End page 196
Total pages 8
Place of publication Basel, Switzerland
Publisher S. Karger AG
Language eng
Formatted abstract
Background/Aims: The polyphenols catechins and theaflavins in black tea have been shown to possess many medicinal properties, including anticancer activity and some antifungal characteristics, but there have been few studies of their anti-Candida activity. In this paper we report the results of our study of the anti-Candida activity of tea polyphenols.

Methods:
The effects of 4 different concentrations of catechins and theaflavins were evaluated on 5 isolates each of 5 Candida species employing an agar diffusion growth inhibition assay. The minimum inhibitory concentration (MIC) of the polyphenols against C. albicans was determined. The post-antifungal effect (PAFE) of the polyphenols for C. albicans was investigated. C. albicans cells exposed to polyphenols were studied using a scanning electron microscope (SEM).

Results:
Both polyphenols showed anti-Candida activity against all tested Candida species and demonstrated a MIC of 6.25 mg/ml for C. albicans. C. glabrata was found to be the most sensitive species followed by C. parapsilosis, C. albicans, C. krusei and C. tropicalis (p < 0.05 for all). Significant intraspecies variations in sensitivity were noted among C. parapsilosis and C. tropicalis (p < 0.001) for both polyphenols. Theaflavins displayed standard PAFE while catechins showed a paradoxical PAFE with all isolates of C. albicans. SEM revealed considerable cell wall damage of C. albicans cells exposed to the polyphenols.

Conclusion: The study reveals for the first time the anti-Candida properties of black tea polyphenols that may find therapeutic applications in future.  
Keyword Antifungal activity
Candida
Post-antifungal effect
Tea polyphenols
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Dentistry Publications
 
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