Actinomyces spp. in supragingival plaque of ethnic Chinese preschool children with and without active dental caries

Tang, G., Yip, H. K., Samaranayake, L. P., Luo, G., Lo, E. C. M. and Teo, C. S. (2003) Actinomyces spp. in supragingival plaque of ethnic Chinese preschool children with and without active dental caries. Caries Research, 37 5: 381-390. doi:10.1159/000072172


Author Tang, G.
Yip, H. K.
Samaranayake, L. P.
Luo, G.
Lo, E. C. M.
Teo, C. S.
Title Actinomyces spp. in supragingival plaque of ethnic Chinese preschool children with and without active dental caries
Formatted title
Actinomyces spp. in supragingival plaque of ethnic Chinese preschool children with and without active dental caries
Journal name Caries Research   Check publisher's open access policy
ISSN 0008-6568
1421-976X
Publication date 2003-09
Year available 2003
Sub-type Article (original research)
DOI 10.1159/000072172
Open Access Status Not yet assessed
Volume 37
Issue 5
Start page 381
End page 390
Total pages 10
Place of publication Basel, Switzerland
Publisher S. Karger AG
Language eng
Formatted abstract
Very limited molecular epidemiological data are available on the role of Actinomyces spp. in the pathogenesis of caries in the primary dentition. Therefore, we investigated their distribution in supragingival plaque of ethnic Chinese preschool children from Singapore and Hong Kong, either with or without active caries. Plaque samples were taken from intact interproximal enamel areas using dental floss. Bacterial genomic DNA of each sample was extracted and variable regions of 16S ribosomal DNA amplified and labelled with digoxigenin. Oligonucleotide probes specific for Actinomyces bovis, Actinomyces gerencseriae, Actinomyces israelii, Actinomyces meyeri, Actinomyces odontolyticus, catalase-negative Actinomyces naeslundii (genospecies 1 and 2) and catalase-positive Actinomyces naeslundii genospecies 2 (previously Actinomyces viscosus serotype II) were used to detect these species using Southern hybridization with a Minislot and Miniblotter system. A. odontolyticus, A. gerencseriae and A. meyeri were detected with similar frequency in both Singapore and Hong Kong samples or in those with and without active caries. However, the prevalence of A. naeslundii was significantly different in the two locales (p < 0.05). A. odontolyticus (88.7%), A. gerencseriae (56.6%) and A. naeslundii (50.9%) were detected in a majority of the samples and the positive hybridization signals of A. gerencseriae in the caries-active group were stronger than from the caries-free group. A. bovis and A. israelii were undetectable in any of the samples. These data imply that A. odontolyticus, A. naeslundii and A. gerencseriae may play an important role in supragingival plaque formation on primary teeth in ethnic Chinese, with others such as A. meyeri contributing. 
Keyword Actinomyces
Chinese preschool children
Dental caries
South-East Asia
Southern hybridization
Supragingival plaque
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Dentistry Publications
MVM records
 
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