Effect of culture media and nutrients on biofilm growth kinetics of laboratory and clinical strains of Enterococcus faecalis

Seneviratne C.J., Yip J.W.Y., Chang J.W.W., Zhang C.F. and Samaranayake L.P. (2013) Effect of culture media and nutrients on biofilm growth kinetics of laboratory and clinical strains of Enterococcus faecalis. Archives of Oral Biology, 58 10: 1327-1334. doi:10.1016/j.archoralbio.2013.06.017


Author Seneviratne C.J.
Yip J.W.Y.
Chang J.W.W.
Zhang C.F.
Samaranayake L.P.
Title Effect of culture media and nutrients on biofilm growth kinetics of laboratory and clinical strains of Enterococcus faecalis
Journal name Archives of Oral Biology   Check publisher's open access policy
ISSN 0003-9969
1879-1506
Publication date 2013-10
Year available 2013
Sub-type Article (original research)
DOI 10.1016/j.archoralbio.2013.06.017
Volume 58
Issue 10
Start page 1327
End page 1334
Total pages 8
Place of publication Oxford United Kingdom
Publisher Pergamon
Collection year 2014
Language eng
Subject 2733 Otorhinolaryngology
1307 Cell Biology
3500 Dentistry
Formatted abstract
Objective: Enterococcus faecalis is a bacterial pathogen that is often associated with endodontic infections. Biofilm formation is a key virulence attribute in the pathogenicity of E. faecalis. In the present study, we comprehensively examined the effect of various culture media and nutrients on the development of E. faecalis biofilms.

Design: A reference strain and a clinical isolate of E. faecalis were used in all experiments for comparison. Commonly used liquid culture media with different nutrient compositions were used to support the development of E. faecalis biofilms in a time-dependent assay. E. faecalis biofilms were quantified by colony forming unit (CFU) and crystal violet (CV) assays. Biofilm architecture and cellular viability were evaluated by scanning electron microscopy and confocal laser scanning microscopy.

Results: Growth kinetics evaluated by CFU and CV assays and by microscopy showed that E. faecalis biofilms reached maturity at 72 h. "Pg broth" (Tryptic Soy Broth with yeast extract, hemen and vitamin K) promoted E. faecalis biofilm formation more than Brain Heart Infusion broth or Tryptic Soy Broth. Addition of 2% glucose enhanced biofilm formation. Thus, it seems that nutrients such as hemen, vitamin K and glucose are important for E. faecalis for the formation of biofilms.

Conclusion: The present study demonstrated that nutrient-rich media containing glucose enhances the formation of E. faecalis biofilms, which exhibit maturation at 72 h.
Keyword Biofilms
Enterococcus faecalis
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
School of Dentistry Publications
 
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