Candida albicans biofilm formation is associated with increased anti-oxidative capacities

Seneviratne, C. Jayampath, Wang, Yu, Jin, Lijian, Abiko, Yoshi and Samaranayake, Lakshman P. (2008) Candida albicans biofilm formation is associated with increased anti-oxidative capacities. Proteomics, 8 14: 2936-2947. doi:10.1002/pmic.200701097


Author Seneviratne, C. Jayampath
Wang, Yu
Jin, Lijian
Abiko, Yoshi
Samaranayake, Lakshman P.
Title Candida albicans biofilm formation is associated with increased anti-oxidative capacities
Journal name Proteomics   Check publisher's open access policy
ISSN 1615-9853
1615-9861
Publication date 2008
Sub-type Article (original research)
DOI 10.1002/pmic.200701097
Open Access Status
Volume 8
Issue 14
Start page 2936
End page 2947
Total pages 12
Place of publication Weinheim, Germany
Publisher Wiley - V C H
Language eng
Abstract Candida albicans is a common, opportunistic, human fungal pathogen that causes a variety of mucosal and systemic afflictions. It exists in nature both in the biofilm or the sessile phase, as well as in the free-floating or the planktonic phase. Candida biofilms, in particular, display unique characteristics that confer survival advantages over their planktonic counterparts, such as their recalcitrance to common antifungals. The mechanisms underlying Candida biofilm formation and their attributes are poorly understood. In this study, we used a 2-DE-based approach to characterize the protein markers that are differentially expressed in Candida biofilms in comparison to their planktonic counterparts. Using tandem mass spectrometric analysis, we have identified a significant number of proteins including alkyl hydroperoxide reductase, thioredoxin peroxidase, and thioredoxin involved in oxidative stress defenses that are upregulated in the biofilm phase. These proteomic findings were further confirmed by real-time PCR and lucigenin-based chemiluminescence assays. In addition, we demonstrate that a drug target for the new antifungal agent echinocandin, is abundantly expressed and significantly upregulated in Candida biofilms. Taken together, these data imply that the biofilm mode, Candida, compared with their planktonic counterparts, exhibits traits that can sustain oxidative stress (anti-oxidants), and thereby exert resistance to commonly used antifungals.
Keyword 2-DE
Anti-oxidant activity
Biofilm
Biomarker
Candida
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Dentistry Publications
 
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