Differential phospholipase gene expression by Candida albicans in artificial media and cultured human oral epithelium

Samaranayake, Y. H., Dassanayake, R. S., Cheung, B. P. K., Jayatilake, J. A. M. S., Yeung, K. W. S., Yau, J. Y. Y. and Samaranayake, L. P. (2006) Differential phospholipase gene expression by Candida albicans in artificial media and cultured human oral epithelium. APMIS, 114 12: 857-866. doi:10.1111/j.1600-0463.2006.apm_479.x


Author Samaranayake, Y. H.
Dassanayake, R. S.
Cheung, B. P. K.
Jayatilake, J. A. M. S.
Yeung, K. W. S.
Yau, J. Y. Y.
Samaranayake, L. P.
Title Differential phospholipase gene expression by Candida albicans in artificial media and cultured human oral epithelium
Formatted title
Differential phospholipase gene expression by Candida albicans in artificial media and cultured human oral epithelium
Journal name APMIS   Check publisher's open access policy
ISSN 0903-4641
1600-0463
Publication date 2006-12
Sub-type Article (original research)
DOI 10.1111/j.1600-0463.2006.apm_479.x
Volume 114
Issue 12
Start page 857
End page 866
Total pages 10
Place of publication Malden, MA, United States
Publisher Wiley-Blackwell Publishing
Language eng
Formatted abstract
Phospholipases B1, B2, C and D of Candida albicans play a significant role in the host invasive process. Hence we evaluated the in vitro expression of PLB1, PLB2, PLC1 and PLD1 in phospholipase-positive (PL+) and -deficient (PL-) C. albicans isolates in egg yolk agar (EYA), yeast peptone dextrose broth (YPD), and in a model of oral candidiasis based on reconstituted human oral epithelium (RHOE). The growth of Candida was then determined in YPD and its cellular invasion was investigated using the RHOE model. The PL+ group demonstrated PLB1, PLB2, PLC1 and PLD1 expression in both EYA and YPD, in contrast to the PL- group, which expressed only PLB2 and PLD1. Although PL+ isolates grew profusely in the RHOE model, they expressed only PLB2, PLC1 and PLD1, and not PLB1. Gene expression investigations could not be carried out with PL- isolates due to their inability to grow in the RHOE model. Significant growth differences in YPD medium were also observed within the PL+ and PL- groups. Taken together, these findings indicate that phospholipase gene expression in C. albicans is differentially affected by their growth milieu, and this in turn may modulate the disease outcomes in vivo.
Keyword C. albicans
Media
Phospholipase gene expression
RHOE
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Dentistry Publications
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