Evaluation of polyene-azole antagonism in liquid cultures of Candida albicans using an automated turbidometric method

Samaranayake, Y. H., Samaranayake, L. P. and Yeung, K. W. S. (2001) Evaluation of polyene-azole antagonism in liquid cultures of Candida albicans using an automated turbidometric method. Chemotherapy, 47 4: 279-291. doi:10.1159/000048535


Author Samaranayake, Y. H.
Samaranayake, L. P.
Yeung, K. W. S.
Title Evaluation of polyene-azole antagonism in liquid cultures of Candida albicans using an automated turbidometric method
Formatted title
Evaluation of polyene-azole antagonism in liquid cultures of Candida albicans using an automated turbidometric method
Journal name Chemotherapy   Check publisher's open access policy
ISSN 0009-3157
1421-9794
Publication date 2001-07
Year available 2001
Sub-type Article (original research)
DOI 10.1159/000048535
Open Access Status Not yet assessed
Volume 47
Issue 4
Start page 279
End page 291
Total pages 13
Place of publication Basel, Switzerland
Publisher S. Karger AG
Language eng
Formatted abstract
Background: A computerized machine, SPECTRAmax 340, was used to evaluate the recently reported phenomenon of antagonism of the polyene amphotericin B (AMB) in Candida albicans pre-exposed to the triazole fluconazole (FLZ).

Methods: We investigated growth inhibition by varying concentrations of AMB in seven isolates of C. albicans pre-exposed to FLZ (50 μg/ml) for 18 h. All isolates were obtained on sequential visits from human immunodeficiency virus-infected patients not treated with FLZ.

Results: Antagonism of AMB activity was observed in 5, 4, 2 and a single isolate for 0.5, 1, 2 and 3 μg/ml of the antifungal, respectively. In the majority of Candida isolates, antagonism was seen within a concentration range of 0.5-1.0 μg/ml AMB; 1 Candida strain (HK1-Sa) was resistant to 3 μg/ml AMB. Higher concentrations of AMB (>3 μg/ml) killed both the controls and FLZ-pre-exposed Candida cells. No significant differences were observed between the periods of antagonism observed for any of the sequential isolates or for any of the AMB concentrations or in the maxima of the growth curves obtained for all Candida isolates.

Conclusion: We conclude that the SPECTRAmax system is a useful tool to evaluate in vitro pharmacodynamic interactions between antifungal regimens within a fluid culture system, and provides information that cannot be obtained using traditional plate assay systems. Copyright
Keyword Amphotericin B
Antagonism
Candida albicans
Fluconazole
Susceptibility
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Dentistry Publications
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