Proteomics of drug resistance in Candida glabrata biofilms

Jayampath Seneviratne, C., Wang, Y., Jin, L., Abiko, Y. and Samaranayake, L. P. (2010) Proteomics of drug resistance in Candida glabrata biofilms. Proteomics, 10 7: 1444-1454. doi:10.1002/pmic.200900611

Author Jayampath Seneviratne, C.
Wang, Y.
Jin, L.
Abiko, Y.
Samaranayake, L. P.
Title Proteomics of drug resistance in Candida glabrata biofilms
Formatted title
Proteomics of drug resistance in Candida glabrata biofilms
Journal name Proteomics   Check publisher's open access policy
ISSN 1615-9853
Publication date 2010
Sub-type Article (original research)
DOI 10.1002/pmic.200900611
Open Access Status
Volume 10
Issue 7
Start page 1444
End page 1454
Total pages 11
Place of publication Weinheim, Germany
Publisher Wiley - V C H Verlag
Language eng
Abstract Candida glabrata is a fungal pathogen that causes a variety of mucosal and systemic infections among compromised patient populations with higher mortality rates. Previous studies have shown that biofilm mode of the growth of the fungus is highly resistant to antifungal agents compared with the free-floating or planktonic mode of growth. Therefore, in the present study, we used 2-D DIGE to evaluate the differential proteomic profiles of C. glabrata under planktonic and biofilm modes of growth. Candida glabrata biofilms were developed on polystyrene surfaces and age-matched planktonic cultures were obtained in parallel. Initially, biofilm architecture, viability, and antifungal susceptibility were evaluated. Differentially expressed proteins more than 1.5-fold in DIGE analysis were subjected to MS/MS. The transcriptomic regulation of these biomarkers was evaluated by quantitative real-time PCR. Candida glabrata biofilms were highly resistant to the antifungals and biocides compared with the planktonic mode of growth. Candida glabrata biofilm proteome when compared with its planktonic proteome showed upregulation of stress response proteins, while glycolysis enzymes were downregulated. Similar trend could be observed at transcriptomic level. In conclusion, C. glabrata biofilms possess higher amount of stress response proteins, which may potentially contribute to the higher antifungal resistance seen in C. glabrata biofilms.
Keyword 2-D DIGE
Candida glabrata
Stress response
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Dentistry Publications
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