Architectural analysis, viability assessment and growth kinetics of Candida albicans and Candida glabrata biofilms

Seneviratne, C. J., Silva, W. J., Jin, L. J., Samaranayake, Y. H. and Samaranayake, L. P. (2009) Architectural analysis, viability assessment and growth kinetics of Candida albicans and Candida glabrata biofilms. Archives of Oral Biology, 54 11: 1052-1060. doi:10.1016/j.archoralbio.2009.08.002


Author Seneviratne, C. J.
Silva, W. J.
Jin, L. J.
Samaranayake, Y. H.
Samaranayake, L. P.
Title Architectural analysis, viability assessment and growth kinetics of Candida albicans and Candida glabrata biofilms
Journal name Archives of Oral Biology   Check publisher's open access policy
ISSN 0003-9969
1879-1506
Publication date 2009
Year available 2009
Sub-type Article (original research)
DOI 10.1016/j.archoralbio.2009.08.002
Open Access Status
Volume 54
Issue 11
Start page 1052
End page 1060
Total pages 9
Place of publication Kidlington, Oxford, United Kingdom
Publisher Pergamon
Collection year 2010
Language eng
Subject 1307 Cell Biology
2733 Otorhinolaryngology
3500 Dentistry
Abstract The human fungal pathogen Candida is able to form biofilms in almost all the medical devices in current use. Indeed, biofilm formation is a major virulence attribute of microorganisms and account for a majority of human infections. Therefore, understanding processes appertaining to biofilm development is an important prerequisite for devising new strategies to prevent or eradicate biofilm-related infections. In the present study we used an array of both conventional and novel analytical tools to obtain a comprehensive view of Candida biofilm development. Enumeration of colony forming units, colorimetric (XTT) assay, Scanning Electron Microscopy (SEM) and novel Confocal Laser Scanning Microscopy (CLSM) coupled with COMSTAT software analyses were utilised to evaluate growth kinetics; architecture and viability of biofilms of a reference (ATCC) and a clinical strain each of two Candida species, C. albicans and C. glabrata. Biofilm growth kinetics on a polystyrene substrate was evaluated from the initial adhesion step (1.5 h) up to 72 h. These analyses revealed substantial inter- and intra-species differences in temporal organisation of Candida biofilm architecture, spatiality and cellular viability, while reaching maturity within a period of 48 h, on a polystyrene substrate. There were substantial differences in the growth kinetics upon methodology, although general trend seemed to be the same. Detailed architectural analysis provided by COMSTAT software corroborated the SEM and CSLM views. These analyses may provide a strong foundation for down stream molecular work of fungal biofilms.
Keyword Architecture
Biofilm
Candida
Growth kinetics
Viability
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Dentistry Publications
 
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