Interspecies variation in Candida biofilm formation studied using the Calgary biofilm device

Parahitiyawa, N. B., Samaranayake, Y. H., Samaranayake, L. P., Ye, J., Tsang, P. W. K., Cheung, B. P. K., Yau, J. Y. Y. and Yeung, S. K. W. (2006) Interspecies variation in Candida biofilm formation studied using the Calgary biofilm device. APMIS, 114 4: 298-306. doi:10.1111/j.1600-0463.2006.apm_394.x


Author Parahitiyawa, N. B.
Samaranayake, Y. H.
Samaranayake, L. P.
Ye, J.
Tsang, P. W. K.
Cheung, B. P. K.
Yau, J. Y. Y.
Yeung, S. K. W.
Title Interspecies variation in Candida biofilm formation studied using the Calgary biofilm device
Formatted title
Interspecies variation in Candida biofilm formation studied using the Calgary biofilm device
Journal name APMIS   Check publisher's open access policy
ISSN 0903-4641
1600-0463
Publication date 2006-04
Sub-type Article (original research)
DOI 10.1111/j.1600-0463.2006.apm_394.x
Open Access Status Not yet assessed
Volume 114
Issue 4
Start page 298
End page 306
Total pages 9
Place of publication Malden, MA, United States
Publisher Wiley-Blackwell Publishing
Language eng
Formatted abstract
An in vitro assay to study multiple Candida biofilms, in parallel, has been carried out using the Calgary biofilm device (CBD). We here report: i) standardization of the CBD for Candida albicans biofilm formation, ii) kinetics of C. albicans biofilm formation, iii) biofilm formation by five Candida species, and iv) effect of dietary carbohydrates on biofilm formation. The biofilm metabolic activity on all CBD pegs was similar (p=0.6693) and C. albicans biofilm formation revealed slow growth up to 36 h and significantly higher growth up to 48 h (p<0.001). Significant differences in total biofilm metabolic activity were seen for glucose, fructose and lactose grown C. albicans compared with sucrose and maltose grown yeasts. Candida krusei developed the largest biofilm mass (p<0.05) relative to C. albicans, C. glabrata, C. dubliniensis and C. tropicalis. Scanning electron microscopy revealed that C. krusei produced a thick multilayered biofilm of pseudohyphal forms embedded within the polymer matrix, whereas C. albicans, C. dubliniensis and C. tropicalis biofilms consisted of clusters or chains of cells with sparse extracellular matrix material. We conclude that CBD is a useful, simple, low cost miniature device for parallel study of Candida biofilms and factors modulating this phenomenon.
Keyword Calgary biofilm device
Candida biofilms
Dietary carbohydrates
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Dentistry Publications
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