Synergistic activity of lysozyme and antifungal agents against Candida albicans biofilms on denture acrylic surfaces

Samaranayake, Y. H., Cheung, B. P. K., Parahitiyawa, N., Seneviratne, C. J., Yau, J. Y. Y., Yeung, K. W. S. and Samaranayake, L. P. (2009) Synergistic activity of lysozyme and antifungal agents against Candida albicans biofilms on denture acrylic surfaces. Archives of Oral Biology, 54 2: 115-126. doi:10.1016/j.archoralbio.2008.09.015

Author Samaranayake, Y. H.
Cheung, B. P. K.
Parahitiyawa, N.
Seneviratne, C. J.
Yau, J. Y. Y.
Yeung, K. W. S.
Samaranayake, L. P.
Title Synergistic activity of lysozyme and antifungal agents against Candida albicans biofilms on denture acrylic surfaces
Journal name Archives of Oral Biology   Check publisher's open access policy
ISSN 0003-9969
Publication date 2009
Year available 2009
Sub-type Article (original research)
DOI 10.1016/j.archoralbio.2008.09.015
Open Access Status
Volume 54
Issue 2
Start page 115
End page 126
Total pages 12
Place of publication Kidlington, Oxford, United Kingdom
Publisher Pergamon
Collection year 2010
Language eng
Subject 1307 Cell Biology
2733 Otorhinolaryngology
3500 Dentistry
Abstract Denture related oral candidiasis is a recalcitrant fungal infection not easily resolved by topical antifungals. The antimycotic protein lysozyme, in saliva is an important host defense mechanism although its activity against Candida biofilms on denture acrylic has not been evaluated. Objectives: (i) To establish a clinically relevant denture acrylic assay model to develop standardized Candida albicans biofilms, and (ii) assess the inhibitory effects of lysozyme alone and, the latter combined with antifungals (nystatin, amphotericin B, ketoconazole and 5-fluorocytosine) on sessile Candida cells and, finally (iii) to visualize the accompanying ultrastructural changes. Design: The rotating-disc biofilm reactor was used to develop standardized 48 h Candida biofilms on acrylic discs in YNB/100 mM glucose medium and the biofilm metabolic activity was monitored using a tetrazolium reduction assay. Results: The biofilm metabolic activity was similar in 18 identical denture acrylic discs (p < 0.05) thus validating the rotating-disc biofilm model. Very low concentrations of lysozyme (6.25 μg/ml) significantly (p < 0.01) inhibited Candida biofilm formation indicating that lysozyme may likely regulate intra-oral Candida biofilm development. Although 100 μg/ml lysozyme killed 45% of sessile Candida cells, further increasing its concentration (up to 240 μg/ml) had no such effect. Nystatin, amphotericin B, and ketoconazole in association with 100 μg/ml lysozyme exhibited effective synergistic killing of biofilm Candida in comparison to drug-free controls. Scanning electron and confocal scanning laser microscopy analysis confirmed the latter trends. Conclusion: Our results indicate that agents found in biological fluids such as lysozyme could be a safe adjunct to antifungals in future treatment strategies for recalcitrant candidal infections.
Keyword Antifungals
Denture acrylic
Rotating disc
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Dentistry Publications
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