Enhancement of Secretory Aspartyl Protease production in biofilms of Candida albicans exposed to sub-inhibitory concentrations of fluconazole

Mores, Alinne Ulbrich, Souza, Roberta Djavana, Cavalca, Laura, de Paula e Carvalho, Alessandra, Gursky, Lauren Christine, Rosa, Rosimeire Takaki, Samaranayake, Lakshman Perera and Ribeiro Rosa, Edvaldo Antonio (2011) Enhancement of Secretory Aspartyl Protease production in biofilms of Candida albicans exposed to sub-inhibitory concentrations of fluconazole. Mycoses, 54 3: 195-201. doi:10.1111/j.1439-0507.2009.01793.x


Author Mores, Alinne Ulbrich
Souza, Roberta Djavana
Cavalca, Laura
de Paula e Carvalho, Alessandra
Gursky, Lauren Christine
Rosa, Rosimeire Takaki
Samaranayake, Lakshman Perera
Ribeiro Rosa, Edvaldo Antonio
Title Enhancement of Secretory Aspartyl Protease production in biofilms of Candida albicans exposed to sub-inhibitory concentrations of fluconazole
Formatted title
Enhancement of Secretory Aspartyl Protease production in biofilms of Candida albicans exposed to sub-inhibitory concentrations of fluconazole
Journal name Mycoses   Check publisher's open access policy
ISSN 0933-7407
1439-0507
Publication date 2011
Sub-type Article (original research)
DOI 10.1111/j.1439-0507.2009.01793.x
Open Access Status
Volume 54
Issue 3
Start page 195
End page 201
Total pages 7
Place of publication Berlin, Germany
Publisher Wiley-Blackwell
Language eng
Abstract The production of Secretory Aspartyl Proteases (Sap) is an important virulence factor of Candida albicans. Many studies have shown that a challenge with sub-inhibitory concentrations of antifungals lead species of Candida to the secretion of higher concentrations of Sap. Nevertheless, published studies only reported the secretion of such enzymes by cells growing in planktonic phase, with few mention of biofilms. The present study evaluated the alterations in the secretion of Sap by C. albicans grown in biofilms and exposed to sub-inhibitory concentrations of fluconazole. The MICs for fluconazole of seven clinical strains were determined for planktonic cells. Biofilm and planktonic cells were grown in the presence of 1/2 MIC, 1/4 MIC, and no medication (control). The relative metabolic activity, indirectly related to cell loads, were estimated by the absorbance of reduced XTT and the Sap activity was evaluated by bovine albumin test. It was observed that 72h-old biofilms under the influence of 1/2 MIC had fewer cells than 1/4 MIC and control. The production of Sap was inversely proportional to the cell content, with higher secretion in 1/2 MIC, followed by 1/4 MIC and control. Biofilms of C. albicans challenged by sub-MICs of fluconazole tend to secrete higher quantities of Sap.
Keyword Aspartyl-protease
Biofilm
Candida albicans
Fluconazole
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Dentistry Publications
 
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