The antimicrobial efficacy of Fructus mume extract on orthodontic bracket: a monospecies-biofilm model study in vitro

Chen, Yong, Wong, Ricky W. K., Seneviratne, C. J., Hagg, Urban, Mcgrath, Colman, Samaranayake, L. P. and Kao, Richard (2011) The antimicrobial efficacy of Fructus mume extract on orthodontic bracket: a monospecies-biofilm model study in vitro. Archives of Oral Biology, 56 1: 16-21. doi:10.1016/j.archoralbio.2010.08.006


Author Chen, Yong
Wong, Ricky W. K.
Seneviratne, C. J.
Hagg, Urban
Mcgrath, Colman
Samaranayake, L. P.
Kao, Richard
Title The antimicrobial efficacy of Fructus mume extract on orthodontic bracket: a monospecies-biofilm model study in vitro
Journal name Archives of Oral Biology   Check publisher's open access policy
ISSN 0003-9969
1879-1506
Publication date 2011-01-01
Sub-type Article (original research)
DOI 10.1016/j.archoralbio.2010.08.006
Volume 56
Issue 1
Start page 16
End page 21
Total pages 6
Place of publication Kidlington, Oxford, United Kingdom
Publisher Pergamon
Language eng
Formatted abstract
Objective: The aim of this study was to evaluate the antimicrobial efficacy of Traditional Chinese Medicine Fructus mume on a monospecies-biofilm model established on orthodontic brackets in vitro.

Materials and methods: The antimicrobial effect of Fructus mume aqueous extract on the planktonic Streptococcus mutans (S. mutans) was tested by microdilution method (MIC). The cell viability of S. mutans biofilm on Damon3 MX bracket (Ormco, USA) after exposed to Fructus mume extract was quantified by XTT reduction assay. Visualization of the samples was performed by fluorescence microscope and confocal laser scanning microscopy (CLSM).

Results: HPLC analysis revealed that the main compounds of Fructus mume are organic acids. The MIC of Fructus mume extract on the planktonic S. mutans was 50 mg/mL. The optical density (OD) values, measured by XTT reduction assay from S. mutans biofilms after 1-min exposure to different test agents, demonstrated that the cell viability of S. mutans biofilms exposed to 250 mg/mL Fructus mume extract < BHI (-) (p < 0.01). Microscope image showed that Fructus mume extract obviously increased the amount of dead bacteria on the surface of bracket.

Conclusion: Fructus mume extract showed antimicrobial effect on S. mutans biofilm on orthodontic bracket in vitro which may indicate its potential use as an oral antimicrobial agent for orthodontic patients.
Keyword Antimicrobial
Fructus mume
Orthodontic
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Dentistry Publications
 
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