Analysis of the hydration water around bovine serum albumin using terahertz coherent synchrotron radiation

Bye, Jordan W, Meliga, Stefano, Ferachou, Denis, Cinque, Gianfelice, Zeitler, J. Axel and Falconer, Robert J. (2014) Analysis of the hydration water around bovine serum albumin using terahertz coherent synchrotron radiation. Journal of Physical Chemistry A, 118 1: 83-88. doi:10.1021/jp407410g


Author Bye, Jordan W
Meliga, Stefano
Ferachou, Denis
Cinque, Gianfelice
Zeitler, J. Axel
Falconer, Robert J.
Title Analysis of the hydration water around bovine serum albumin using terahertz coherent synchrotron radiation
Journal name Journal of Physical Chemistry A   Check publisher's open access policy
ISSN 1089-5639
1520-5215
Publication date 2014-01-09
Year available 2013
Sub-type Article (original research)
DOI 10.1021/jp407410g
Open Access Status
Volume 118
Issue 1
Start page 83
End page 88
Total pages 6
Place of publication Washington, DC, United States
Publisher American Chemical Society
Collection year 2014
Language eng
Abstract Terahertz spectroscopy was used to study the absorption of bovine serum albumin (BSA) in water. The Diamond Light Source operating in a low alpha mode generated coherent synchrotron radiation that covered a useable spectral bandwidth of 0.3-3.3 THz (10-110 cm-1). As the BSA concentration was raised, there was a nonlinear change in absorption inconsistent with Beer's law. At low BSA concentrations (0-1 mM), the absorption remained constant or rose slightly. Above a concentration of 1 mM BSA, a steady decrease in absorption was observed, which was followed by a plateau that started at 2.5 mM. Using a overlapping hydration layer model, the hydration layer was estimated to extend 15 Å from the protein. Calculation of the corrected absorption coefficient (αcorr) for the water around BSA by subtracting the excluded volume of the protein provides an alternative approach to studying the hydration layer that provides evidence for complexity in the population of water around BSA.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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