Deciphering hematopoietic stem cells in their niches: a critical appraisal of genetic models, lineage tracing, and imaging strategies

Joseph, Chacko, Quach, Julie M., Walkley, Carl R., Lane, Steven W., Lo Celso, Cristina and Purton, Louise E. (2013) Deciphering hematopoietic stem cells in their niches: a critical appraisal of genetic models, lineage tracing, and imaging strategies. Cell Stem Cell, 13 5: 520-533. doi:10.1016/j.stem.2013.10.010


Author Joseph, Chacko
Quach, Julie M.
Walkley, Carl R.
Lane, Steven W.
Lo Celso, Cristina
Purton, Louise E.
Title Deciphering hematopoietic stem cells in their niches: a critical appraisal of genetic models, lineage tracing, and imaging strategies
Journal name Cell Stem Cell   Check publisher's open access policy
ISSN 1934-5909
1875-9777
Publication date 2013-11
Year available 2013
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1016/j.stem.2013.10.010
Open Access Status
Volume 13
Issue 5
Start page 520
End page 533
Total pages 14
Place of publication Cambridge, MA, United States
Publisher Cell Press
Collection year 2014
Language eng
Abstract In recent years, technical developments in mouse genetics and imaging equipment have substantially advanced our understanding of hematopoietic stem cells (HSCs) and their niche. The availability of numerous Cre strains for targeting HSCs and microenvironmental cells provides extensive flexibility in experimental design, but it can also pose significant challenges due to strain-specific differences in cell specificity. Here we outline various genetic approaches for isolating, detecting, and ablating HSCs and niche components and provide a guide for advantages and caveats to consider. We also discuss opportunities and limitations presented by imaging technologies that allow investigation of HSC behavior in situ.
Keyword Laser scanning cytometry
Site-specific recombination
Diphtheria-toxin receptor
Bone marrow niche
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collections: Official 2014 Collection
School of Medicine Publications
 
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