Nonradioactive in situ hybridization: optimization for tissue sections from pregnant uteri and placenta during the first half of pregnancy

Bany, Brent M. and Simmons, David G. (2014). Nonradioactive in situ hybridization: optimization for tissue sections from pregnant uteri and placenta during the first half of pregnancy. In B. Anne Croy, Aureo T. Yamada, Francesco J. DeMayo and S. Lee Adamson (Ed.), The Guide to Investigation of Mouse Pregnancy (pp. 591-604) London, UK: Academic Press. doi:10.1016/B978-0-12-394445-0.00050-3


Author Bany, Brent M.
Simmons, David G.
Title of chapter Nonradioactive in situ hybridization: optimization for tissue sections from pregnant uteri and placenta during the first half of pregnancy
Title of book The Guide to Investigation of Mouse Pregnancy
Place of Publication London, UK
Publisher Academic Press
Publication Year 2014
Sub-type Chapter in textbook
DOI 10.1016/B978-0-12-394445-0.00050-3
Open Access Status
Year available 2013
ISBN 9780123944450
9780123947949
Editor B. Anne Croy
Aureo T. Yamada
Francesco J. DeMayo
S. Lee Adamson
Chapter number 50
Start page 591
End page 604
Total pages 14
Total chapters 71
Collection year 2014
Language eng
Formatted Abstract/Summary
Nonradioactive in situ hybridization is a simple technique with a large number of steps that can be customized to the varying needs of individual researchers. It permits the localization of one mRNA or simultaneously two mRNAs in tissue sections from the peri-implantation uterus and developing placenta. By varying the detection methods, use of the protocols presented here results in either colorimetric or fluorescent localization of the mRNA(s) of interest, yielding useful data concerning the precise localization of gene expression at the maternal–conceptus interface.
Keyword Digoxigenin
In situ hybridization
Riboprobes
Q-Index Code BX
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 9 December 2013

Document type: Book Chapter
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Created: Fri, 31 Jan 2014, 18:45:22 EST by David Simmons on behalf of School of Biomedical Sciences