Tuneable “Nano-Shearing”: a physical mechanism to displace nonspecific cell adhesion during rare cell detection

Vaidyanathan, Ramanathan, Shiddiky, Muhammad J. A., Rauf, Sakandar, Dray, Eloise, Tay, Zhikai and Trau, Matt (2014) Tuneable “Nano-Shearing”: a physical mechanism to displace nonspecific cell adhesion during rare cell detection. Analytical Chemistry, 86 4: 2042-2049. doi:10.1021/ac4032516


Author Vaidyanathan, Ramanathan
Shiddiky, Muhammad J. A.
Rauf, Sakandar
Dray, Eloise
Tay, Zhikai
Trau, Matt
Title Tuneable “Nano-Shearing”: a physical mechanism to displace nonspecific cell adhesion during rare cell detection
Formatted title
Tuneable “Nano-Shearing”: a physical mechanism to displace nonspecific cell adhesion during rare cell detection
Journal name Analytical Chemistry   Check publisher's open access policy
ISSN 0003-2700
1520-6882
Publication date 2014-01-22
Sub-type Article (original research)
DOI 10.1021/ac4032516
Open Access Status
Volume 86
Issue 4
Start page 2042
End page 2049
Total pages 8
Place of publication Washington, DC, United States
Publisher American Chemical Society
Collection year 2015
Language eng
Formatted abstract
We report a tuneable alternating current electro-hydrodynamic (ac-EHD) force which drives lateral fluid motion within a few nanometers of an electrode surface. Because the magnitude of this fluid shear force can be tuned externally (e.g., via the application of an ac electric field), it provides a new capability to physically displace weakly (nonspecifically) bound cellular analytes. To demonstrate the utility of the tuneable nanoshearing phenomenon, we present data on purpose-built microfluidic devices that employ ac-EHD to remove nonspecific adsorption of molecular and cellular species. Here we show that an ac-EHD device containing asymmetric planar and microtip electrode pairs resulted in a 4-fold reduction in nonspecific adsorption of blood cells, and also capture breast cancer cells in blood, with high efficiency (approximately 87%) and specificity. We therefore feel that this new capability of externally tuning and manipulating fluid flow could have wide applications as an innovative approach to enhance the specific capture of rare cells such as cancer cells in blood.
Keyword AC electrohydrodynamics
Shear forces
Nonspecific adsorption
Cell capture
Microfluidic devices
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Fri, 31 Jan 2014, 10:22:24 EST by Mr Sakandar Rauf on behalf of Aust Institute for Bioengineering & Nanotechnology