Viewing golgi structure and function from a different perspective-insights from electron tomography

Marsh, Brad J. and Pavelka, Margit (2013). Viewing golgi structure and function from a different perspective-insights from electron tomography. In Methods for analysis of Golgi complex function (pp. 259-279) San Diego, CA, United States: Academic Press (Elsevier). doi:10.1016/B978-0-12-417164-0.00016-1


Author Marsh, Brad J.
Pavelka, Margit
Title of chapter Viewing golgi structure and function from a different perspective-insights from electron tomography
Title of book Methods for analysis of Golgi complex function
Place of Publication San Diego, CA, United States
Publisher Academic Press (Elsevier)
Publication Year 2013
Sub-type Research book chapter (original research)
DOI 10.1016/B978-0-12-417164-0.00016-1
Series Methods in Cell Biology
ISBN 9780124171640
ISSN 0091-679X
Volume number 118
Chapter number 16
Start page 259
End page 279
Total pages 21
Total chapters 24
Collection year 2014
Subjects 1307 Cell Biology
Abstract/Summary Historically, ultrastructural investigations, which have focused on elucidating the biological idiosyncrasies of the Golgi apparatus, have tended towards oversimplified or fallacious hypotheses when postulating how the Golgi apparatus reorganizes itself both structurally and functionally to fulfill the plethora of cellular processes underpinned by this complex organelle. Key questions are still unanswered with regard to how changes in Golgi architecture correlate so reproducibly to changes in its functional priorities under different physiological conditions or experimental perturbations. This fact alone serves to highlight how the technical limitations associated with conventional two-dimensional imaging approaches employed in the past failed to adequately capture the extraordinary complexity of the Golgi's three-dimensional (3D) structure-now a hallmark of this challenging organelle. Consequently, this has hampered progress towards developing a clear understanding of how changes in its structure and function typically occur in parallel. In this chapter, we highlight but a few of the significant new insights regarding variations in the Golgi's structure-function relationships that have been afforded over recent years through advanced electron microscopic techniques for 3D image reconstruction, commonly referred to as electron tomography.
Keyword 3D reconstruction
Electron microscopy
Electron tomography
Endocytosis
Golgi apparatus
Membrane traffic
Protein transport
Q-Index Code B1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Thu, 30 Jan 2014, 23:02:15 EST by Anthony Yeates on behalf of Examinations