RNA extraction from developing or mature wheat seeds

Furtado, Agnelo (2014). RNA extraction from developing or mature wheat seeds. In Robert J. Henry and Agnelo Furtado (Ed.), Cereal Genomics: Methods and Protocols (pp. 23-28) New York, NY, United States: Humana Press. doi:10.1007/978-1-62703-715-0


Author Furtado, Agnelo
Title of chapter RNA extraction from developing or mature wheat seeds
Title of book Cereal Genomics: Methods and Protocols
Place of Publication New York, NY, United States
Publisher Humana Press
Publication Year 2014
Sub-type Chapter in reference work, encyclopaedia, manual or handbook
DOI 10.1007/978-1-62703-715-0
Open Access Status
Year available 2013
Series Methods in Molecular Biology
ISBN 9781627037143
1627037152
9781627037150
ISSN 1064-3745
1940-6029
Editor Robert J. Henry
Agnelo Furtado
Volume number 1099
Chapter number 4
Start page 23
End page 28
Total pages 6
Total chapters 24
Collection year 2014
Language eng
Subjects 1312 Molecular Biology
1311 Genetics
Abstract/Summary Cereal grains, as storage tissues of the plant, contain high amounts of starch. Purification of RNA from plant tissue especially from seed tissue can be challenging due to this high starch content. Starch coprecipitates with RNA in the presence of isopropanol or ethanol and can interfere with the extraction process and downstream reactions. Thus the removal of starch by using appropriate methods is necessary for obtaining pure RNA to be processed for functional genomics analysis. We describe a method to isolate large amount of good-quality RNA from developing and mature wheat grain which can also be adapted to other cereal grains.
Keyword Cereal
Extraction
Grain
Starch
Q-Index Code BX
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Tue, 28 Jan 2014, 00:23:03 EST by System User on behalf of Centre for Nutrition and Food Sciences