Protein blotting protocol for beginners

Petrasovits, Lars A. (2014). Protein blotting protocol for beginners. In Robert J. Henry and Agnelo Furtado (Ed.), Cereal genomics: methods and protocols (pp. 189-199) New York, NY United States: Humana Press, Inc.. doi:10.1007/978-1-62703-715-0


Author Petrasovits, Lars A.
Title of chapter Protein blotting protocol for beginners
Title of book Cereal genomics: methods and protocols
Place of Publication New York, NY United States
Publisher Humana Press, Inc.
Publication Year 2014
Sub-type Chapter in reference work, encyclopaedia, manual or handbook
DOI 10.1007/978-1-62703-715-0
Open Access Status
Year available 2013
Series Methods in Molecular Biology
ISBN 9781627037143
1627037152
9781627037150
ISSN 1064-3745
1940-6029
Editor Robert J. Henry
Agnelo Furtado
Volume number 1099
Chapter number 16
Start page 189
End page 199
Total pages 11
Total chapters 24
Collection year 2014
Language eng
Subjects 1312 Molecular Biology
1311 Genetics
Abstract/Summary The transfer and immobilization of biological macromolecules onto solid nitrocellulose or nylon (polyvinylidene difluoride (PVDF)) membranes subsequently followed by specific detection is referred to as blotting. DNA blots are called Southerns after the inventor of the technique, Edwin Southern. By analogy, RNA blots are referred to as northerns and protein blots as westerns (Burnette, Anal Biochem 112:195-203, 1981). With few exceptions, western blotting involves five steps, namely, sample collection, preparation, separation, immobilization, and detection. In this chapter, protocols for the entire process from sample collection to detection are described.
Keyword Electrophoretic transfer
Immunodetection
Protein extraction
Trizol
Q-Index Code BX
Q-Index Status Confirmed Code
Institutional Status UQ

 
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