Isolation and molecular identification of Avibacterium paragallinarum in suspected cases of infectious coryza

Askari Badouei, Mahdi, Sadrzadeh, Avesta, Azad, Nader, Blackall, Patrick, Madadgar, Omid and Charkhkar, Saeid (2013) Isolation and molecular identification of Avibacterium paragallinarum in suspected cases of infectious coryza. Turkish Journal of Veterinary and Animal Sciences, 38 1: 46-49. doi:10.3906/vet-1301-62


Author Askari Badouei, Mahdi
Sadrzadeh, Avesta
Azad, Nader
Blackall, Patrick
Madadgar, Omid
Charkhkar, Saeid
Title Isolation and molecular identification of Avibacterium paragallinarum in suspected cases of infectious coryza
Formatted title
Isolation and molecular identification of Avibacterium paragallinarum in suspected cases of infectious coryza
Journal name Turkish Journal of Veterinary and Animal Sciences   Check publisher's open access policy
ISSN 1300-0128
1303-6181
Publication date 2013-01-01
Sub-type Article (original research)
DOI 10.3906/vet-1301-62
Open Access Status DOI
Volume 38
Issue 1
Start page 46
End page 49
Total pages 4
Place of publication Ankara, Turkey
Publisher Scientific and Technical Research Council of Turkey
Collection year 2014
Language eng
Subject 3400 Veterinary
Formatted abstract
Isolation and identification of Avibacterium paragallinarum, the causative agent of infectious coryza, is considered a challenging task in laboratories with limited specialties. In the present study, 14 commercial layer fowls showing the typical symptoms of infectious coryza were subjected to primary isolation followed by polymerase chain reaction confirmation of suspect colonies (culture-PCR). Direct PCR assays on infraorbital sinus swab samples were also carried out. Thirty-five suspected cases of infectious coryza in commercial broiler chickens were also screened using direct PCR on infraorbital sinus swabs. In culture-PCR, only 1 of the 4 suspected isolates was confirmed as Av. paragallinarum. In comparison, in direct PCR, 5 layer samples were shown to be positive for Av. paragallinarum. All of the broiler samples were negative in the direct PCR assay. Our findings indicate that primary isolation in combination with PCR can be a simple method for diagnosis of infectious coryza, although with a lower sensitivity than direct PCR. While direct PCR is comparably the more rapid and sensitive test, there will be instances in which the bacterial isolate is needed for further use. Hence, the culture-PCR method can be a practical and simple approach, especially in laboratories with limited specialty in identification of this fastidious organism.
Keyword Avibacterium paragallinarum
Culture-PCR
Direct PCR
Infectious coryza
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Alliance for Agriculture and Food Innovation
Official 2014 Collection
 
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