High-throughput sequencing and mutagenesis to accelerate the domestication of Microlaena stipoides as a new food crop

Shapter, Frances M., Cross, Michael, Ablett, Gary, Malory, Sylvia, Chivers, Ian H., King, Graham J. and Henry, Robert J. (2013) High-throughput sequencing and mutagenesis to accelerate the domestication of Microlaena stipoides as a new food crop. PLoS One, 8 12: e82641.1-e82641.9. doi:10.1371/journal.pone.0082641


Author Shapter, Frances M.
Cross, Michael
Ablett, Gary
Malory, Sylvia
Chivers, Ian H.
King, Graham J.
Henry, Robert J.
Title High-throughput sequencing and mutagenesis to accelerate the domestication of Microlaena stipoides as a new food crop
Formatted title
High-throughput sequencing and mutagenesis to accelerate the domestication of Microlaena stipoides as a new food crop
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2013-12
Sub-type Article (original research)
DOI 10.1371/journal.pone.0082641
Open Access Status DOI
Volume 8
Issue 12
Start page e82641.1
End page e82641.9
Total pages 9
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Collection year 2014
Language eng
Formatted abstract
Global food demand, climatic variability and reduced land availability are driving the need for domestication of new crop species. The accelerated domestication of a rice-like Australian dryland polyploid grass, Microlaena stipoides (Poaceae), was targeted using chemical mutagenesis in conjunction with high throughput sequencing of genes for key domestication traits. While M. stipoides has previously been identified as having potential as a new grain crop for human consumption, only a limited understanding of its genetic diversity and breeding system was available to aid the domestication process. Next generation sequencing of deeply-pooled target amplicons estimated allelic diversity of a selected base population at 14.3 SNP/Mb and identified novel, putatively mutation-induced polymorphisms at about 2.4 mutations/Mb. A 97% lethal dose (LD97) of ethyl methanesulfonate treatment was applied without inducing sterility in this polyploid species. Forward and reverse genetic screens identified beneficial alleles for the domestication trait, seed-shattering. Unique phenotypes observed in the M2 population suggest the potential for rapid accumulation of beneficial traits without recourse to a traditional cross-breeding strategy. This approach may be applicable to other wild species, unlocking their potential as new food, fibre and fuel crops.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Alliance for Agriculture and Food Innovation
Official 2014 Collection
 
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Citation counts: TR Web of Science Citation Count  Cited 13 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 15 times in Scopus Article | Citations
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