FLT3-ligand treatment of humanized mice results in the generation of large numbers of CD141+ and CD1c+ dendritic cells in vivo

Ding, Yitian, Wilkinson, Andrew, Idris, Adi, Fancke, Ben, O’Keeffe, Meredith, Khalil, Dalia, Ju, Xinsheng, Lahoud, Mireille H., Caminschi, Irina, Shortman, Ken, Rodwell, Robyn, Vuckovic, Slavica and Radford, Kristen J. (2014) FLT3-ligand treatment of humanized mice results in the generation of large numbers of CD141+ and CD1c+ dendritic cells in vivo. Journal of Immunology, . doi:10.4049/​jimmunol.1302391


Author Ding, Yitian
Wilkinson, Andrew
Idris, Adi
Fancke, Ben
O’Keeffe, Meredith
Khalil, Dalia
Ju, Xinsheng
Lahoud, Mireille H.
Caminschi, Irina
Shortman, Ken
Rodwell, Robyn
Vuckovic, Slavica
Radford, Kristen J.
Title FLT3-ligand treatment of humanized mice results in the generation of large numbers of CD141+ and CD1c+ dendritic cells in vivo
Journal name Journal of Immunology   Check publisher's open access policy
ISSN 0022-1767
1550-6606
Publication date 2014-01-22
Sub-type Article (original research)
DOI 10.4049/​jimmunol.1302391
Total pages 8
Place of publication Bethesda, MD, United States
Publisher American Association of Immunologists
Collection year 2015
Language eng
Abstract We established a humanized mouse model incorporating FLT3-ligand (FLT3-L) administration after hematopoietic cell reconstitution to investigate expansion, phenotype, and function of human dendritic cells (DC). FLT3-L increased numbers of human CD141+ DC, CD1c+ DC, and, to a lesser extent, plasmacytoid DC (pDC) in the blood, spleen, and bone marrow of humanized mice. CD1c+ DC and CD141+ DC subsets were expanded to a similar degree in blood and spleen, with a bias toward expansion of the CD1c+ DC subset in the bone marrow. Importantly, the human DC subsets generated after FLT3-L treatment of humanized mice are phenotypically and functionally similar to their human blood counterparts. CD141+ DC in humanized mice express C-type lectin-like receptor 9A, XCR1, CADM1, and TLR3 but lack TLR4 and TLR9. They are major producers of IFN-λ in response to polyinosinic-polycytidylic acid but are similar to CD1c+ DC in their capacity to produce IL-12p70. Although all DC subsets in humanized mice are efficient at presenting peptide to CD8+ T cells, CD141+ DC are superior in their capacity to cross-present protein Ag to CD8+ T cells following activation with polyinosinic-polycytidylic acid. CD141+ DC can be targeted in vivo following injection of Abs against human DEC-205 or C-type lectin-like receptor 9A. This model provides a feasible and practical approach to dissect the function of human CD141+ and CD1c+ DC and evaluate adjuvants and DC-targeting strategies in vivo.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Biomedical Sciences Publications
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Created: Fri, 24 Jan 2014, 10:32:25 EST by Anthony Yeates on behalf of School of Medicine