Characterisation of oxidized recombinant human galectin-1

Scott, Stacy A., Bugarcic, Andrea and Blanchard, Helen (2009) Characterisation of oxidized recombinant human galectin-1. Protein and Peptide Letters, 16 10: 1249-1255. doi:10.2174/092986609789071342


Author Scott, Stacy A.
Bugarcic, Andrea
Blanchard, Helen
Title Characterisation of oxidized recombinant human galectin-1
Journal name Protein and Peptide Letters   Check publisher's open access policy
ISSN 0929-8665
1875-5305
Publication date 2009
Year available 2009
Sub-type Article (original research)
DOI 10.2174/092986609789071342
Volume 16
Issue 10
Start page 1249
End page 1255
Total pages 7
Place of publication Bussum, The Netherlands
Publisher Bentham Science Publishers Ltd.
Collection year 2010
Language eng
Formatted abstract
Oxidized human galectin-1 plays a role in the immune response to injured axons. Over-expression of galectin-1 by cancer, in combination with cancer associated oxidative stress suggests oxidized human galectin-1 may also play a role(s) in tumourigenesis. Here we generate milligram quantities of oxidized human galectin-1 and undertake biophysical characterization. The protein adopts a number of different states. Two separable oxidized forms are identified that exist as largely mono-disperse solutions at higher milligram/ml concentrations. The presence of disulphide bonds is confirmed for these two protein forms, as is their change in overall shape and loss of lectin activity. Our studies lead to production of a particular mono-disperse oxidized human galectin-1 species that is anticipated most optimal for investigations requiring milligram/ml concentrations such as X-ray crystallography.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biomedical Sciences Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 11 times in Thomson Reuters Web of Science Article | Citations
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Created: Thu, 23 Jan 2014, 07:11:26 EST by Andrea Bugarcic on behalf of School of Biomedical Sciences