Genomic evolution of the pathogenic Wolbachia strain, wMelPop

Woolfit, Megan, Iturbe-Ormaetxe, Inaki, Brownlie, Jeremy C., Walker, Thomas, Riegler, Markus, Seleznev, Andrei, Popovici, Jean, Rances, Edwige, Wee, Bryan A., Pavlides, Jennifer, Sullivan, Mitchell J., Beatson, Scott A., Lane, Amanda, Sidhu, Manpreet, McMeniman, Conor J., McGraw, Elizabeth A. and O'Neill, Scott L. (2013) Genomic evolution of the pathogenic Wolbachia strain, wMelPop. Genome Biology and Evolution, 5 11: 2189-2204. doi:10.1093/gbe/evt169


Author Woolfit, Megan
Iturbe-Ormaetxe, Inaki
Brownlie, Jeremy C.
Walker, Thomas
Riegler, Markus
Seleznev, Andrei
Popovici, Jean
Rances, Edwige
Wee, Bryan A.
Pavlides, Jennifer
Sullivan, Mitchell J.
Beatson, Scott A.
Lane, Amanda
Sidhu, Manpreet
McMeniman, Conor J.
McGraw, Elizabeth A.
O'Neill, Scott L.
Title Genomic evolution of the pathogenic Wolbachia strain, wMelPop
Formatted title
Genomic evolution of the pathogenic Wolbachia strain, wMelPop
Journal name Genome Biology and Evolution   Check publisher's open access policy
ISSN 1759-6653
Publication date 2013-01
Year available 2013
Sub-type Article (original research)
DOI 10.1093/gbe/evt169
Open Access Status DOI
Volume 5
Issue 11
Start page 2189
End page 2204
Total pages 16
Place of publication Oxford, United Kingdom
Publisher Oxford University Press
Collection year 2014
Language eng
Formatted abstract
Most strains of the widespread endosymbiotic bacterium Wolbachia pipientis are benign or behave as reproductive parasites. The pathogenic strain wMelPop is a striking exception, however: it overreplicates in its insect hosts and causes severe life shortening. The mechanism of this pathogenesis is currently unknown. We have sequenced the genomes of three variants of wMelPop and of the closely related nonpathogenic strain wMelCS. We show that the genomes of wMelCS and wMelPop appear to be identical in the nonrepeat regions of the genome and differ detectably only by the triplication of a 19-kb region that is unlikely to be associated with life shortening, demonstrating that dramatic differences in the host phenotype caused by this endosymbiont may be the result of only minor genetic changes. We also compare the genomes of the original wMelPop strain from Drosophila melanogaster and two sequential derivatives, wMelPop-CLA and wMelPop-PGYP. To develop wMelPop as a novel biocontrol agent, it was first transinfected into and passaged in mosquito cell lines for approximately 3.5 years, generating wMelPop-CLA. This cell line-passaged strain was then transinfected into Aedes aegypti mosquitoes, creating wMelPop-PGYP, which was sequenced after 4 years in the insect host. We observe a rapid burst of genomic changes during cell line passaging, but no further mutations were detected after transinfection into mosquitoes, indicating either that host preadaptation had occurred in cell lines, that cell lines are a more selectively permissive environment than animal hosts, or both. Our results provide valuable data on the rates of genomic and phenotypic change in Wolbachia associated with host shifts over short time scales.
Keyword Wolbachia
Evolution
Endosymbiont
Genomics
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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