Age-associated differential production of IFN-γ, IL-10 and GM-CSF by porcine alveolar macrophages in response to lipopolysaccharide

Islam, Mohammad Ariful, Uddin, Muhammad Jasim, Tholen, Ernst, Tesfaye, Dawit, Looft, Christian, Schellander, Karl and Cinar, Mehmet Ulas (2013) Age-associated differential production of IFN-γ, IL-10 and GM-CSF by porcine alveolar macrophages in response to lipopolysaccharide. Veterinary Journal, 198 1: 245-251. doi:10.1016/j.tvjl.2013.07.026


Author Islam, Mohammad Ariful
Uddin, Muhammad Jasim
Tholen, Ernst
Tesfaye, Dawit
Looft, Christian
Schellander, Karl
Cinar, Mehmet Ulas
Title Age-associated differential production of IFN-γ, IL-10 and GM-CSF by porcine alveolar macrophages in response to lipopolysaccharide
Journal name Veterinary Journal   Check publisher's open access policy
ISSN 1090-0233
1532-2971
Publication date 2013
Sub-type Article (original research)
DOI 10.1016/j.tvjl.2013.07.026
Open Access Status
Volume 198
Issue 1
Start page 245
End page 251
Total pages 7
Place of publication London, United Kingdom
Publisher Elsevier
Collection year 2014
Abstract The aim of the present study was to investigate the age-related production variation of T helper (Th)-type cytokines (IL-2, IL-4, IFN-γ and IL-10), granulocyte macrophage-colony stimulating factor (GM-CSF) and nitric oxide (NO) by lipopolysaccharide (LPS)-stimulated porcine alveolar macrophages (AMs) in a time-dependent manner. For this purpose, AMs were isolated from 5-days (newborn), 40-days (post-weaned) and 120-days (young) old pigs. Cells were incubated for 24. h in the absence or presence of increasing concentrations of LPS (0.0, 0.01, 1.0, 5.0 and 10.0 μg/mL).IL-10, IFN-γ and GM-CSF mRNA expression was upregulated in a dose-dependent manner for all age groups (P< 0.05). Age-related differences included a significantly increased IL-10 mRNA and protein production in newborn piglets compared to post-weaned and young pigs. IL-10 production pattern was similar with a higher peak between 12 and 36. h post-induction in all age groups. In contrast, IFN-γ mRNA and protein level was significantly elevated in young pigs 12. h and 24. h post-induction, respectively, while the time course production of IFN-γ was mostly consistent in newborn and post-weaned piglets. GM-CSF mRNA expression was significantly lower in newborn piglets than in post-weaned and young pigs. The kinetic of GM-CSF expression peaked at 12. h in young and post-weaned pigs and at 24. h in newborn piglets. IL-4 mRNA levels were very low and no apparent change of IL-2 expression was observed following LPS stimulation in all age groups. Only very low levels of NO were detected in the cell supernatants of young pigs. Collectively, these studies suggest age-related differences in time-dependent production of IL-10, IFN-γ and GM-CSF by porcine AMs with potential immunoregulatory consequences to be explored further.
Keyword Alveolar macrophage
Cytokine
Immune system
Lipopolysaccharide
Piglet
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
School of Veterinary Science Publications
 
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Created: Wed, 15 Jan 2014, 10:03:19 EST by Ms Kate Rowe on behalf of School of Veterinary Science