Proapoptotic and antimetastatic properties of supercritical CO2 extract of Nigella sativa Linn. Against breast cancer cells

Baharetha, Hussein M., Nassar, Zeyad D., Aisha, Abdalrahim F., Ahamed, Mohamed B. Khadeer, Al-Suede, Foaud Saleih R., Kadir, Mohd Omar Abd, Ismail, Zhari and Majid, Amin Malik Shah Abdul (2013) Proapoptotic and antimetastatic properties of supercritical CO2 extract of Nigella sativa Linn. Against breast cancer cells. Journal of Medicinal Food, 16 12: 1121-1130. doi:10.1089/jmf.2012.2624

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Author Baharetha, Hussein M.
Nassar, Zeyad D.
Aisha, Abdalrahim F.
Ahamed, Mohamed B. Khadeer
Al-Suede, Foaud Saleih R.
Kadir, Mohd Omar Abd
Ismail, Zhari
Majid, Amin Malik Shah Abdul
Title Proapoptotic and antimetastatic properties of supercritical CO2 extract of Nigella sativa Linn. Against breast cancer cells
Formatted title
Proapoptotic and antimetastatic properties of supercritical CO2 extract of Nigella sativa Linn. Against breast cancer cells
Journal name Journal of Medicinal Food   Check publisher's open access policy
ISSN 1096-620X
1557-7600
Publication date 2013-12
Year available 2013
Sub-type Article (original research)
DOI 10.1089/jmf.2012.2624
Open Access Status File (Author Post-print)
Volume 16
Issue 12
Start page 1121
End page 1130
Total pages 10
Place of publication New Rochelle, United States
Publisher Mary Ann Liebert
Collection year 2014
Language eng
Formatted abstract
Nigella sativa, commonly referred as black cumin, is a popular spice that has been used since the ancient Egyptians. It has traditionally been used for treatment of various human ailments ranging from fever to intestinal disturbances to cancer. This study investigated the apoptotic, antimetastatic, and anticancer activities of supercritical carbon dioxide (SC-CO2) extracts of the seeds of N. sativa Linn. against estrogen-dependent human breast cancer cells (MCF-7). Twelve extracts were prepared from N. sativa seeds using the SC-CO2 extraction method by varying pressure and temperature. Extracts were analyzed using FTIR and UV-Vis spectrometry. Cytotoxicity of the extracts was evaluated on various human cancer and normal cell lines. Of the 12 extracts, 1 extract (A3) that was prepared at 60 C and 2500 psi (∼17.24 MPa) showed selective antiproliferative activity against MCF-7 cells with an IC 50 of 53.34±2.15 μg/mL. Induction of apoptosis was confirmed by evaluating caspases activities and observing the cells under a scanning electron microscope. In vitro antimetastatic properties of A3 were investigated by colony formation, cell migration, and cell invasion assays. The elevated levels of caspases in A3 treated MCF-7 cells suggest that A3 is proapoptotic. Further nuclear condensation and fragmentation studies confirmed that A3 induces cytotoxicity through the apoptosis pathway. A3 also demonstrated remarkable inhibition in migration and invasion assays of MCF-7 cells at subcytotoxic concentrations. Thus, this study highlights the therapeutic potentials of SC-CO2 extract of N. sativa in targeting breast cancer.
Keyword Caspases
Hoechst 33258
MCF-7 cells
MTT
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Pharmacy Publications
 
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