Evaluation of different methods for determination of the iron saturation level in bovine lactoferrin

Bokkhim, Huma, Tran, Trang, Bansal, Nidhi, Grondahl, Lisbeth and Bhandari, Bhesh (2014) Evaluation of different methods for determination of the iron saturation level in bovine lactoferrin. Food Chemistry, 152 121-127. doi:10.1016/j.foodchem.2013.11.132


Author Bokkhim, Huma
Tran, Trang
Bansal, Nidhi
Grondahl, Lisbeth
Bhandari, Bhesh
Title Evaluation of different methods for determination of the iron saturation level in bovine lactoferrin
Journal name Food Chemistry   Check publisher's open access policy
ISSN 0308-8146
1873-7072
Publication date 2014-06-01
Year available 2013
Sub-type Article (original research)
DOI 10.1016/j.foodchem.2013.11.132
Open Access Status
Volume 152
Start page 121
End page 127
Total pages 7
Place of publication Amsterdam, The Netherlands
Publisher Elsevier
Collection year 2014
Language eng
Abstract Lactoferrin (Lf) samples with ca. 25%, 50%, 75%, 85% and 100% iron saturation were prepared for the purpose of evaluating Chromametry, Differential Scanning Calorimetry (DSC) and Circular Dichroism (CD) spectropolarimetry for their suitability in determining the iron saturation level. Numerical values for colour from Chromametry, enthalpy change of denaturation (ΔH cal) from DSC and molar ellipticities from CD were statistically analysed to evaluate their correlation with the level of iron saturation in Lf. Linear regression analysis of colour coordinates Chroma (C â̂ -) and hue (h) angle on percentage iron saturation level of Lf showed that the values can be used to estimate the iron saturation level. The ΔHcal for the iron saturated peak and the CD ellipticities in the 310-340 nm region provided reliable data for the estimation of iron saturation level of Lf up to 75%. Mono- and di-saturated Lf displayed the same thermal stability and very similar tertiary structures.
Keyword Colour space parameters
Ellipticity
Enthalpy of denaturation
Iron saturation
Lactoferrin
Polar coordinates
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Official 2014 Collection
School of Chemistry and Molecular Biosciences
 
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