Evaluation of silk sericin as a biomaterial: in vitro growth of human corneal limbal epithelial cells on Bombyx mori sericin membranes

Chirila, Traian V., Suzuki, Shuko, Bray, Laura J., Barnett, Nigel L. and Harkin, Damien G. (2013) Evaluation of silk sericin as a biomaterial: in vitro growth of human corneal limbal epithelial cells on Bombyx mori sericin membranes. Progress in Biomaterials, 2 14: 1-10. doi:10.1186/2194-0517-2-14

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Author Chirila, Traian V.
Suzuki, Shuko
Bray, Laura J.
Barnett, Nigel L.
Harkin, Damien G.
Title Evaluation of silk sericin as a biomaterial: in vitro growth of human corneal limbal epithelial cells on Bombyx mori sericin membranes
Journal name Progress in Biomaterials   Check publisher's open access policy
ISSN 2194-0517
Publication date 2013-11-28
Sub-type Article (original research)
DOI 10.1186/2194-0517-2-14
Open Access Status DOI
Volume 2
Issue 14
Start page 1
End page 10
Total pages 10
Place of publication Heidelberg, Germany
Publisher SpringerOpen
Collection year 2014
Language eng
Formatted abstract
Sericin and fibroin are the two major proteins in the silk fibre produced by the domesticated silkworm, Bombyx mori. Fibroin has been extensively investigated as a biomaterial. We have previously shown that fibroin can function successfully as a substratum for growing cells of the eye. Sericin has been so far neglected as a biomaterial because of suspected allergenic activity. However, this misconception has now been dispelled, and sericin’s biocompatibility is currently indisputable. Aiming at promoting sericin as a possible substratum for the growth of corneal cells in order to make tissue-engineered constructs for the restoration of the ocular surface, in this study we investigated the attachment and growth in vitro of human corneal limbal epithelial cells (HLECs) on sericin-based membranes. Sericin was isolated and regenerated from the silkworm cocoons by an aqueous procedure, manufactured into membranes, and characterized (mechanical properties, structural analysis, contact angles). Primary cell cultures from two donors were established in serum-supplemented media in the presence of murine feeder cells. Membranes made of sericin and fibroin-sericin blends were assessed in vitro as substrata for HLECs in a serum-free medium, in a cell attachment assay and in a 3-day cell growth experiment. While the mechanical characteristics of sericin were found to be inferior to those of fibroin, its ability to enhance the attachment of HLECs was significantly superior to fibroin, as revealed by the PicoGreen® assay. Evidence was also obtained that cells can grow and differentiate on these substrata.
Keyword Silk
Silk proteins
Sericin
Corneal limbal epithelial cells
Cell attachment
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Mon, 16 Dec 2013, 10:38:55 EST by Roheen Gill on behalf of Faculty Of Health Sciences