Association and expression study of MMP3, TGF beta 1 and COL10A1 as candidate genes for leg weakness-related traits in pigs

Laenoi, Watchara, Rangkasenee, Noppawan, Uddin, Muhammad Jasim, Cinar, Mehmet Ulas, Phatsara, Chirawath, Tesfaye, Dawit, Scholz, Armin M., Tholen, Ernst, Looft, Christian, Mielenz, Manfred, Sauerwein, Helga, Wimmers, Klaus and Schellander, Karl (2012) Association and expression study of MMP3, TGF beta 1 and COL10A1 as candidate genes for leg weakness-related traits in pigs. Molecular Biology Reports, 39 4: 3893-3901. doi:10.1007/s11033-011-1168-5


Author Laenoi, Watchara
Rangkasenee, Noppawan
Uddin, Muhammad Jasim
Cinar, Mehmet Ulas
Phatsara, Chirawath
Tesfaye, Dawit
Scholz, Armin M.
Tholen, Ernst
Looft, Christian
Mielenz, Manfred
Sauerwein, Helga
Wimmers, Klaus
Schellander, Karl
Title Association and expression study of MMP3, TGF beta 1 and COL10A1 as candidate genes for leg weakness-related traits in pigs
Journal name Molecular Biology Reports   Check publisher's open access policy
ISSN 0301-4851
1573-4978
Publication date 2012-04
Sub-type Article (original research)
DOI 10.1007/s11033-011-1168-5
Volume 39
Issue 4
Start page 3893
End page 3901
Total pages 9
Place of publication Dordrecht, Netherlands
Publisher Springer
Language eng
Abstract The present study was aimed to determine the association between metalloproteinase 3 (MMP3), transforming growth factor beta 1 (TGFb1) and collagen type X alpha I (COL10A1) gene polymorphisms with traits related to leg weakness in pigs. Three hundred Duroc 9 Pietrain cross breds (DuPi) and 299 pigs of a commercial population (CP) were used for the experiment. DuPi animals were examined for 10 different traits describing leg and feet structure, osteochondrosis (OC) scores and bone density status. Data of OC score at condylus medialis humeri, condylus medialis femoris and distal epiphysis ulna regions of CP were used for association analysis. Significant association (P<0.05) was found for MMP3 SNP (g.158 C>T) with OC at head of femur and bone mineral density in the DuPi population. Association (P<0.05) was found between SNP of TGFb1 (g.180 G>A) with rear leg score and the principle component denoting both OC and feet and leg scores in the DuPi population. No association was found between COL10A1 (g.72 C>T) and leg weakness related traits. The associations of SNPs with OC traits could not be confirmed in the commercial population. Expression analysis of the three candidate genes was performed to compare between healthy and OC. TGFb1 was found to be highly expressed (P<0.05) in the OC compared to healthy cartilages, but no significant different expressions were observed for MMP3 and COL10A1 genes. The present finding suggested that TGFb1 and MMP3 genes variants have an effect on some of the leg weakness related traits.
Keyword Leg weakness
Osteochondrosis
Gene polymorphism
Association
Pig
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Veterinary Science Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 5 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 6 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Fri, 13 Dec 2013, 18:52:42 EST by System User on behalf of School of Veterinary Science